基于网络药理学探讨大黄-水蛭-香附配伍治疗子宫内膜异位症的作用机制  

Mechanism of Rhubarb-Leech-Incense Compatibility in the Treatment of Endometriosis Based on Network Pharmacology

作　　者：邓颖[1] 刘金坤[2] 白殊同[2] DENG Ying;LIU Jinkun;BAI Shutong(Dept.of Gynecology,Chongqing Hospital of Traditional Chinese Medicine,Chongqing 400021,China;Chongqing Key Laboratory of Traditional Chinese Medicine to Prevent and Treat Autoimmune Diseases,Chongqing Hospital of Traditional Chinese Medicine,Chongqing 400021,China)

机构地区：[1]重庆市中医院妇科,重庆400021 [2]重庆市中医院中医药防治自身免疫疾病重庆市重点实验室,重庆400021

出　　处：《中国医院用药评价与分析》2022年第2期142-146,共5页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：重庆市卫生健康委员会中医药科研项目青年项目(No.2021ZY4108);重庆市科学技术局科研院所绩效激励引导专项项目(No.cstc2018jxjl130034);重庆市科学技术局科研机构绩效激励引导专项项目(No.cstc2017jxjl130041)。

摘　　要：目的:采用网络药理方法研究大黄-水蛭-香附治疗子宫内膜异位症的分子机制。方法:采用中药系统药理学数据库与分析平台和BATMAN-TCM数据库,筛选大黄-水蛭-香附配伍的活性成分和靶点信息。通过对比治疗靶点数据库和GeneCards平台获得子宫内膜异位症疾病靶基因信息,使用Venny软件对大黄-水蛭-香附配伍靶基因和子宫内膜异位症靶基因取交集,获得药物作用核心靶点。通过Cytoscape软件构建"成分-靶点-疾病"网络,并通过STRING数据库分析核心靶点蛋白质-蛋白质相互作用。通过R语言对核心靶点进行基因本体(GO)功能富集分析,通过DAVID数据库进行京都基因与基因组百科全书(KEGG)通路富集分析。结果:共筛选到大黄-水蛭-香附配伍的38个活性成分和98个潜在核心靶点,其中槲皮素、木犀草素、山柰酚、番红花酸和乌索酸为主要化学成分,PTGS2、AR、PPARG、TOP2A和ESR1为主要靶点。大黄-水蛭-香附配伍影响的主要生物过程包括脂多糖应答、甾体激素应答和活性氧应答,KEGG通路富集分析得到330条信号通路,主要包含磷脂酰肌醇-3-激酶/蛋白激酶B(PI3K-Akt)、缺氧诱导因子(HIF)和肿瘤坏死因子(TNF)信号通路。结论:大黄-水蛭-香附的可通过山柰酚、槲皮素和木犀草素等成分与PTGS2、AR和ESR1等靶点发生相互作用,调节PI3K-Akt、HIF和TNF等多信号通路,影响细胞功能、氧化应答和免疫调节等一系列生物过程,进而实现治疗子宫内膜异位症的作用。OBJECTIVE:To study the mechanism of rhubarb-leech-incense compatibility in treatment of endometriosis(EMT)based on network pharmacology.METHODS:TCMSP and BATMAN-TCM database were used to screen the active ingredients and target information of rhubarb-leech-incense.The target gene information of endometriosis was obtained through the treatment target database and GeneCards platform.Venny software was used to analyze the intersection of rhubarb-leeches-rhizome compatibility target gene and endometriosis target gene to obtain the core target of drugs.The“ingredient-target-disease”network was constructed by Cytoscape software,and the core target protein-protein interactions were analyzed by STRING database.Gene ontology(GO)functional enrichment analysis of core targets was performed by R software,and pathway enrichment analysis of Kyoto Encyclopedia of Genes and Genomes(KEGG)was performed by DAVID database.RESULTS:A total of 38 active ingredients and 98 potential core targets were screened.Quercetin,luteolin,kaempferol,safflower acid and ursolic acid were the main chemical constituents,and PTGS2,AR,PPARG,TOP2 A and ESR1 were the main targets.The main biological processes affected by rhubarb-leech-incense compatibility included lipopolysaccharide response,steroid hormone response and reactive oxygen species response.KEGG pathway enrichment analysis revealed 330 signaling pathways,mainly including PI3 K-Akt,HIF and TNF signaling pathways.CONCLUSIONS:Rhubarb-leech-incense can interact with PTGS2,AR and ESR1 through kaempferol,quercetin and luteolin to regulate PI3 K-Akt,HIF and TNF and other signaling pathways,affecting cell function,oxidative response and immune regulation,thus achieving a series of biological processes for the treatment of endometriosis.

关 键 词：子宫内膜异位症 大黄 水蛭 香附 网络药理学 药理机制 

分 类 号：R932[医药卫生—生药学] R96[医药卫生—药学]