线粒体DNA拷贝数与燃煤污染型地方性氟中毒的关联分析  

Correlation between mitochondrial DNA copy number and coal-burning-borne endemic fluorosis

作　　者：赵安宿 田薇 喻艳琴 李瑞超 王婵娟[1] 张婷[1] 单可人[1] 何燕[1] Zhao Ansu;Tian Wei;Yu Yanqin;Li Ruichao;Wang Chanjuan;Zhang Ting;Shan Keren;He Yan(Key Laboratory of Molecular Biology of Guizhou Province,Key Laboratory of Endemic and Ethnic Diseases,Ministry of Education,Guizhou Medical University,Guiyang 550004,China)

机构地区：[1]贵州医科大学地方病与少数民族疾病教育部重点实验室,贵州省医学分子生物学重点实验室,贵阳550004

出　　处：《中华地方病学杂志》2022年第2期111-116,共6页Chinese Journal of Endemiology

基　　金：贵州省科技支撑计划项目(黔科合支撑(2019)2807)。

摘　　要：目的探讨相对线粒体DNA拷贝数(mitochondrial DNA copy number,mtDNA-CN)与燃煤污染型地方性氟中毒(简称燃煤型地氟病)的关联。方法2018年6月至2019年3月,采用横断面研究,在贵州省典型燃煤型地氟病病区毕节市选择482例燃煤型地氟病患者作为病例组;同时,在非病区长顺县选择212例健康人群作为对照组。通过问卷调查和体格检查收集两组人群基本信息、生活行为习惯等一般情况,采集外周静脉血血样,采用实时荧光定量PCR法检测外周血相对mtDNA-CN;应用二分类和无序多分类logistic回归等方法分析相对mtDNA-CN与燃煤型地氟病的相关性。结果对照组与病例组人群的体质指数(body mass index,BMI)及性别比例、婚姻状况、文化程度分布情况比较差异均有统计学意义(t=7.91,χ2=5.11、13.33、34.32,P均<0.05);且对照组人群相对mtDNA-CN高于病例组[中位数(四分位数):202(138,292)比131(96,217),Z=-7.80,P<0.001]。二分类logistic回归分析结果[比值比(95%置信区间)]显示,文化程度[小学:0.572(0.377~0.868)、初中及以上:0.292(0.174~0.493)]、相对mtDNA-CN[131~<217:0.265(0.144~0.488)、≥217:0.183(0.100~0.335)]、BMI[1.222(1.142~1.307)]均为燃煤型地氟病患病风险的影响因素(P均<0.05)。在不同BMI及文化程度亚组中,相对mtDNA-CN与燃煤型地氟病患病风险均呈显著负相关(P趋势均<0.05);相对mtDNA-CN与人群BMI、文化程度对燃煤型地氟病均不存在交互效应(P交互均>0.05)。无序多分类logistic回归分析结果显示,相对mtDNA-CN与氟斑牙、氟骨症的患病风险均呈显著负相关(P趋势均<0.05)。结论相对mtDNA-CN越高,人群燃煤型地氟病患病风险越低,提示mtDNA-CN可能是燃煤型地氟病的潜在生物标志物。Objective To investigate the association between relative mitochondrial DNA copy number(mtDNA-CN)and coal-burning-borne endemic fluorosis(abbreviated as coal-burning-borne fluorosis).Methods From June 2018 to March 2019,using cross-sectional study,482 patients with coal-burning-borne fluorosis were selected as the case group in Bijie City,a typical coal-burning-borne fluorosis area of Guizhou Province;meanwhile,212 healthy individuals from Changshun County,a non-coal-burning-borne fluorosis area in Guizhou Province,were selected as the control group.Questionnaire survey and physical examination were used to collect general condition such as basic information and living habits of the two groups,peripheral venous blood samples were collected,and real-time fluorescence quantitative PCR was used to detect the relative mtDNA-CN in peripheral blood.The correlation between relative mtDNA-CN and coal-burning-borne fluorosis was analyzed by binary and unordered multi-class logistic regression.Results There were significant differences in the body mass index(BMI),and the distribution of gender rario,marital status and education level between the control group and the case group(t=7.91,χ2=5.11,13.33,34.32,P<0.05).The relative mtDNA-CN in the control group was higher than that in the case group[median(quartile):202(138,292)vs 131(96,217),Z=-7.80,P<0.001].The results of binary logistic regression analysis[odds ratio(95%confidence interval)]showed that educational level[primary school:0.572(0.377-0.868),junior high school and above:0.292(0.174-0.493)],relative mtDNA-CN[131-<217:0.265(0.144-0.488),≥217:0.183(0.100-0.335)]and BMI[1.222(1.142-1.307)]were the influencing factors for the risk of coal-burning-borne fluorosis(P<0.05).In subgroups with different BMI and educational levels,the relative mtDNA-CN was significantly negatively correlated with the risk of coal-burning-borne fluorosis(Ptrend<0.05),and there was no interaction between mtDNA-CN and BMI and educational levels(Pinteraction>0.05).The results of unordered multi

关 键 词：氟 煤 线粒体DNA 拷贝数 横断面研究 影响因素 

分 类 号：R599.1[医药卫生—内科学]