镉胁迫对艾纳香抗性生理、亚细胞镉分布和镉化学形态的影响  

Effects of Cadmium Stress on Resistant Physiology,Sbcellular Distribution and Chemical Forms of Cadmium in Blumea balsamifera

作　　者：陈子涵 任建国[1] 庞玉新 王俊丽[1] CHEN Zihan;REN Jianguo;PANG Yuxin;WANG Junli(School of Public Health,the Key Laboratory of Enviromental Pollution Monitoring and Disease Control,Ministry of Education,Guizhou Medical University,Guizhou Provincial Engineering Research Center of Food Nutrition and Health,Guiyang 550025,China;School of Chinese Materia Resources,Guangdong Pharmaceutical University,Yunfu Guangdong 527500,China)

机构地区：[1]贵州医科大学公共卫生与健康学院,环境污染与疾病监控教育部重点实验室,贵州省食品营养与健康工程研究中心,贵阳550025 [2]广东药科大学中药资源学院,广东云浮527500

出　　处：《种子》2022年第2期31-39,共9页Seed

基　　金：国家自然科学基金(31960265);贵州省区域内一流学科建设项目-公共卫生与预防医学(黔教科研发[2017]85号);贵州省高等学校工程研究中心项目(黔教合KY字[2021]008)资助

摘　　要：为探讨镉胁迫下艾纳香耐镉机制,采用盆栽试验,研究土壤镉(Cd)(0、0.5、5 mg/kg)胁迫下艾纳香各器官的抗性生理、亚细胞Cd分布和化学形态的变化情况。结果表明,随着外源Cd处理浓度的增加,艾纳香各器官Cd含量均增加,且表现为叶>茎>根;地上部Cd富集系数表现为0.5 mg/kg处理组(8.16)>对照组(2.40)>5 mg/kg处理组(1.65),Cd转运系数表现为对照组(2.35)>5 mg/kg处理组(1.90)>0.5 mg/kg处理组(1.79)。艾纳香各器官非蛋白巯基化合物总量表现为茎>叶>根。各器官超氧化物歧化酶(SOD)和过氧化物酶(POD)活性均随着外源Cd处理浓度的增加呈现先升后降的趋势。各器官亚细胞Cd相对比率均表现为细胞壁>可溶组分>细胞器,且含量随着Cd处理浓度的增加而增加。在Cd胁迫下,各器官Cd化学形态主要以迁移力、毒性处于中间水平的氯化钠提取态和迁移力弱、毒性小的醋酸提取态为主。本研究结果表明,艾纳香通过非蛋白巯基化合物的螯合作用、植株抗氧化作用以及细胞壁沉积和Cd化学形态的改变达到解毒作用。To investigate the cadmium-resistant mechanism of Blumea balsamifera under cadmium stress,pot experiments were conducted to study the changes of resistant physiology,subcellular Cd-distribution and chemical forms of each organ under different cadmium(Cd)stress(0,0.5 and 5 mg/kg).The results showed that with the increase of the concentration of exogenous Cd,the content of Cd in all organs of B.balsamifera increased in the order of leaf>stem>root.The enrichment coefficient of Cd in shoot followed as treatment group with 0.5 mg/kg Cd(8.16)>control group(2.40)>treatment group with 5 mg/kg Cd(1.65),and the transport coefficient of Cd was as follows control group(2.35)>treatment group with 5 mg/kg Cd(1.90)>treatment group with 0.5 mg/kg Cd(1.79).The total amount of non-protein thiols(NPT)in all organs of B.balsamifera was the highest in stems,followed by leaves and the lowest in roots.The activities of superoxide dismutase(SOD)and peroxidase(POD)in all organs increased firstly and then decreased with the increase of exogenous Cd treatment concentration,and the relative ratio of subcellular Cd in all organs was cell wall>soluble component>organelle,and the content of subcellular Cd increased with the increase of Cd concentration.Under the stress of Cd,the chemical forms of Cd in each organ mainly consisted of the NaCl-extractable state with middle level of transfer capacity and virulence and acetic acid-extractable state with low level of transfer capacity and virulence.The results of this study indicated that the detoxification of B.balsamifera was achieved through chelation of NPT,plant antioxidant,cell wall deposition and change of chemical forms of Cd.

关 键 词：镉胁迫 艾纳香 抗性生理 亚细胞组分 化学形态 

分 类 号：S567[农业科学—中草药栽培]