卵巢早衰病人血清微小RNA-125b表达及与中医证型的相关性分析  

Analysis of correlations between serum microRNA-125b and TCM syndromes in patients with premature ovarian failure

作　　者：李海霞[1] 李慧朋 王文文[3] LI Haixia;LI Huipeng;WANG Wenwen(Department of Gynecology,The 7th People's Hospital of Zhengzhou,Zhengzhou,Henan 450016,China;Department of Traditional Chinese Medicine,The 7th People's Hospital of Zhengzhou,Zhengzhou,Henan 450016,China;Department of Pharmacy,The 7th People's Hospital of Zhengzhou,Zhengzhou,Henan 450016,China)

机构地区：[1]郑州市第七人民医院妇科,河南郑州450016 [2]郑州市第七人民医院中医科,河南郑州450016 [3]郑州市第七人民医院药学部,河南郑州450016

出　　处：《安徽医药》2022年第3期509-513,共5页Anhui Medical and Pharmaceutical Journal

基　　金：2018年度河南省科技攻关项目(182102310237)。

摘　　要：目的探讨卵巢早衰病人血清微小RNA(miR)-125b表达水平及与中医证型的相关性分析。方法选取2017年3月至2019年6月郑州市第七人民医院妇科收治的136例卵巢早衰病人作为研究组,选取同期120例该院体检健康者作为对照组,观察两组研究对象及不同中医证型血清miR-125b、抗米勒管激素(AMH)、卵泡刺激素(FSH)/黄体生成素(LH)水平变化,分析miR-125b表达水平与AMH、FSH/LH、中医证型的关系。结果卵巢早衰病人以肾虚肝郁证(41.18%)、肝肾阴虚证(23.53%)、肾虚血瘀证(16.91%)、脾肾阳虚证(14.70%)较为常见;研究组病人血清AMH水平[(1.98±0.64)pmol/L比(10.95±2.62)pmol/L]明显低于对照组(P<0.05),miR-125b[(2.67±0.91)比(1.01±0.29)]、FSH[(47.28±9.15)U/L比(6.05±1.02)U/L]、LH[(30.46±8.24)U/L比(5.32±1.37)U/L]、FSH/LH比值[(1.62±0.52)比(1.05±0.31)]明显高于对照组(P<0.05);肝肾阴虚证组病人血清AMH水平低于肾虚肝郁证、肾虚血瘀证、脾肾阳虚证病人(P<0.05),脾肾阳虚证组低于肾虚血瘀证组(P<0.05);肝肾阴虚证组病人miR-125b、FSH、LH水平高于肾虚肝郁证、肾虚血瘀证、脾肾阳虚证病人(P<0.05),脾肾阳虚证组miR-125b、FSH、LH水平高于肾虚血瘀证、肾虚肝郁证组(P<0.05);肝肾阴虚证组病人FSH/LH比值低于肾虚肝郁证、肾虚血瘀证病人(P<0.05),脾肾阳虚证组低于肾虚肝郁证病人(P<0.05);卵巢早衰病人血清miR-125b表达水平与AMH水平呈负相关(P<0.05),与FSH/LH比值呈正相关(P<0.05);血清AMH与肾虚肝郁证、肝肾阴虚证、脾肾阳虚证呈负相关(P<0.05),miR-125b、FSH/LH比值与肾虚肝郁证、肝肾阴虚证、肾虚血瘀证、脾肾阳虚证呈正相关(P<0.05)。结论卵巢早衰病人血清miR-125b高表达,与AMH水平呈负相关,与FSH/LH比值呈正相关,且肝肾阴虚证、脾肾阳虚证病人血清miR-125b水平变化较为明显,血清AMH与肝肾阴虚证、脾肾阳虚证呈负相关。Objective To investigate the expression level of serum microRNA(miR)-125b in patients with premature ovarian failure(POF)and its correlation with traditional Chinese medicine syndromes.Methods A total of 136 patients with POF who were admitted to the Department of Gynecology of the 7th People's Hospital of Zhengzhou from March 2017 to June 2019 were selected for the study,and 120 healthy people with physical examination in the hospital during the same period were selected as the control group.Changes in serum miR-125b,anti-Mullerian hormone(AMH),follicle-stimulating hormone(FSH)/luteinizing hormone(LH)in the two groups and different TCM syndromes were observed,and the correlations between miR-125b and AMH,FSH/LH,and TCM syndromes were ana⁃lyzed.Results In patients with POF patients,kidney deficiency and liver stasis syndrome(41.18%),Yang deficiency of liver and kid⁃ney syndrome(23.53%),kidney deficiency and blood stasis syndrome(16.91%)and Yang deficiency of spleen and kidney syndrome(14.70%)were more common;the serum AMH level in patients in the study group was significantly lower than that in the control group[(1.98±0.64)pmol/L vs.(10.95±2.62)pmol/L](P<0.05),while miR-125b[(2.67±0.91)pmol/L vs.(1.01±0.29)pmol/L],FSH[(47.28±9.15)pmol/L vs.(6.05±1.02)pmol/L],LH[(30.46±8.24)pmol/L vs.(5.32±1.37)pmol/L]levels and the FSH/LH ratio[(1.62±0.52)pmol/L vs.(1.05±0.31)pmol/L]was significantly higher than that of the control group(P<0.05);the serum AMH level of the patients with Yang deficiency of liver and kidney syndrome was lower than that of patients with kidney deficiency and liver stasis syndrome,kidney deficiency and blood stasis syndrome,and Yang deficiency of spleen and kidney syndrome(P<0.05),and the serum AMH level of the patients with kidney deficiency and blood stasis syndrome was lower than that of the patients with Yang deficiency of spleen and kidney syndrome(P<0.05);the levels of miR-125b,FSH,and LH in patients with Yang deficiency of liver and kidney syndrome were higher than those in patien

关 键 词：原发性卵巢功能不全 微小RNA-125b 中医证型 相关性 

分 类 号：R271.9[医药卫生—中西医结合]