新疆地区VEGF +936C/T和-634G/C基因多态性与子痫前期和妊娠期糖尿病的相关性分析  

Correlation analysis of VEGF+936C/T and-634G/C gene polymorphisms with pre-eclampsia and gestational diabetes mellitus in Xinjiang

作　　者：陈红艳 李刚 薛淑媛 纪少云 肖磊 颜桦[1] CHEN Hongyan;LI Gang;XUE Shuyuan;JI Shaoyun;XIAO Lei;YAN Hua(the National Engineering Research Center for Miniaturized Detection Systems,Northwest University,Xi'an 710069;Xi'an Fourth Hospital,Xi'an 710004;Urumqi Maternal and Child Health Care Hospital,Urumqi 830001,China)

机构地区：[1]西北大学国家微检测系统工程技术研究中心,陕西西安710069 [2]西安市第四医院,陕西西安710004 [3]乌鲁木齐市妇幼保健院,新疆乌鲁木齐830001

出　　处：《临床医学研究与实践》2022年第7期1-5,共5页Clinical Research and Practice

基　　金：陕西省科技厅2020年陕西省自然科学基础研究计划项目(No.2020JQ-958)。

摘　　要：目的探讨血管内皮生长因子(VEGF)两个单核苷酸多态性位点(+936C/T和-634G/C)与新疆地区常见的妊娠并发症(子痫前期和妊娠期糖尿病)发病风险的相关性。方法共招募2102例女性受试者,包括1551例正常妊娠孕妇(正常组)、232例子痫前期患者(子痫前期组)和319例妊娠期糖尿病患者(妊娠期糖尿病组)。用Sanger测序技术进行VEGF+936C/T和-634G/C基因分型检测。用t检验、Pearson卡方检验和无条件Logistic回归分析进行临床特征和基因分型结果的统计分析。结果在任何遗传模式下均未观察到VEGF+936C/T和-634G/C基因型频率在子痫前期组、妊娠期糖尿病组与正常组间存在差异(P>0.05)。无条件Logistic回归(调整年龄、BMI、民族、产次和月经不调等混杂因素)分析结果表明,VEGF+936C/T和-634G/C多态性不是子痫前期和妊娠期糖尿病的独立危险因素(P>0.05)。结论本研究是首个评估新疆人群VEGF基因型/单倍型与子痫前期和妊娠期糖尿病可能联系的病例-对照研究,结果显示,新疆地区VEGF+936C/T和-634G/C基因多态性与子痫前期和妊娠期糖尿病的发生均无显著相关性。Objective To investigate the correlation of two single nucleotide polymorphisms(+936C/T and-634G/C)of vascular endothelial growth factor(VEGF)with the risk of common complications during pregnancy(pre-eclampsia and gestational diabetes mellitus)in Xinjiang.Methods A total of 2102 female subjects were recruited,including 1551 healthy pregnant women(normal group),232 cases of pre-eclampsia(pre-eclampsia group)and 319 cases of gestational diabetes mellitus(gestational diabetes mellitus group).VEGF+936C/T and-634G/C genotypes were detected by Sanger sequencing technology.The t-test,Pearson chi-square test and unconditional Logistic regression analysis were used to analyze the clinical characteristics and genotyping results.Results No difference was observed in the frequencies of VEGF+936C/T and-634G/C genotypes between pre-eclampsia group,gestational diabetes mellitus group and normal group under any model of inheritance(P>0.05).The unconditional Logistic regression(adjusted for confounding factors such as age,BMI,national,parity and irregular menstruation)analysis result showed that the+936C/T and-634G/C polymorphisms were not independent risk factors for pre-eclampsia and gestational diabetes mellitus(P>0.05).Conclusion This study is the first case-control study to assess the possible association between VEGF genotype/haplotype and pre-eclampsia and gestational diabetes mellitus in Xinjiang population.The results shows that there is no significant correlation of VEGF+936C/T and-634G/C gene polymorphisms with the occurrence of pre-eclampsia and gestational diabetes mellitus in Xinjiang.

关 键 词：血管内皮生长因子 基因多态性 子痫前期 妊娠期糖尿病 

分 类 号：R714.2[医药卫生—妇产科学]