白细胞介素-22基因多态性与小儿支气管哮喘易感性的关系分析  

Relationship between interleukin-22 gene polymorphism and susceptibility to bronchial asthma in children

作　　者：王艳[1] 杨德彬 WANG Yan;YANG Debin(Department of Emergency Medicine,Children’s Hospital of Zhengzhou University,Zhengzhou 450000,China)

机构地区：[1]郑州大学附属儿童医院急诊医学科,河南郑州450000

出　　处：《安徽医学》2022年第3期284-289,共6页Anhui Medical Journal

基　　金：河南省医学科技攻关计划联合共建项目(项目编号:LHGJ20190913)。

摘　　要：目的探究白细胞介素-22(IL-22)基因多态性与小儿支气管哮喘易感性的关系。方法选择2019年6月至2021年6月郑州大学附属儿童医院收治的86例支气管哮喘患儿(研究组)以及同期在该院体检的74例健康儿童(对照组)作为研究对象。两组对象均测定IL-22基因的3个SNP位点(rs1179251、rs2227484、rs2227485)多态性以及血清中IL-22、IL-22受体(IL-22R)、IL-10受体(IL-10R)水平,分析IL-22基因多态性与支气管哮喘易感性、血清指标以及临床表型(中性粒细胞表型、嗜酸性粒细胞表型)的关系。结果研究组与对照组儿童rs1179251、rs2227484、rs2227485位点基因型频率分布符合Hardy-Weinbery平衡;研究组儿童rs1179251位点CC、CT基因型、rs2227485位点GG基因型检出率高于对照组(P<0.05);rs1179251位点CT基因型、T等位基因,rs2227485位点GA基因型可能与哮喘发病风险降低有关(OR=0.589,95%CI:0.378~0.918;OR=0.658,95%CI:0.456~0.950;OR=0.658,95%CI:0.466~0.929);研究组儿童血清IL-22、IL-22R水平高于对照组,rs1179251位点CC基因型、rs2227485位点GG基因型与血清IL-22、IL-22R水平有关(P<0.05);嗜酸性粒细胞表型组儿童rs1179251位点CC、CT基因型、rs2227485位点GG基因型检出率高于中性粒细胞表型组(P<0.05),血清IL-22、IL-22R水平高于中性粒细胞表型组(P<0.05)。结论IL-22基因rs1179251位点、rs2227485位点多态性可能与小儿支气管哮喘易感、血清IL-22水平以及临床表型有关。Objective To explore the relationship between interleukin-22(IL-22)gene polymorphism and susceptibility to bronchial asthma in children.Methods Eighty-six children with bronchial asthma admitted to the Children’s Hospital of Zhengzhou University between June 2019 and June 2021 were selected as the study group.Meanwhile,74 healthy children were selected as the control group.The polymorphisms of 3 SNP loci(rs1179251,rs2227484,rs2227485)on IL-22 gene,and serum levels of IL-22,IL-22 receptor(IL-22R)andIL-10 receptor(IL-10R)in the two groups were determined.The relationship of IL-22 gene polymorphism with susceptibility to bronchial asthma,serum indicators and clinical phenotypes(neutrophil phenotype and eosinophil phenotype)was analyzed.Results The genotypefrequency distributionat rs1179251,rs2227484 and rs2227485 in both groups was in accordance with Hardy-Weinbery equilibrium.The detection rates of genotype CC and CT at rs1179251,and genotype GG at rs2227485 in the study group were significantly higher than those in the control group(P<0.05).Genotype CT and allele T at rs1179251,and genotype GA at rs2227485 might be associated with the the risk of asthma(OR=0.589,95%CI:0.378~0.918;OR=0.658,95%CI:0.456~0.950;OR=0.658,95%CI:0.466~0.929).The serum levels of IL-22 and IL-22R in the study group were higher than those in the control group.Genotype CC at rs1179251 and genotype GG at rs2227485 were related to serum IL-22 and IL-22R levels(P<0.05).The detection rates of genotype CC and CT at rs1179251,and genotype GG at rs2227485 in the eosinophil phenotype group were higher than those in the neutrophil phenotype group(P<0.05).Meanwhile,serum IL-22 and IL-22R levels in the eosinophil phenotype group were higher than those in the neutrophil phenotype group(P<0.05).Conclusion IL-22 rs1179251 and rs2227485 polymorphisms may be related to the susceptibilityto bronchial asthma,serum IL-22 level and clinical phenotypes in children.

关 键 词：白细胞介素-22 基因多态性 小儿支气管哮喘 

分 类 号：R725.6[医药卫生—儿科]