广西蔗区甘蔗白条病菌的鉴定与致病力分析  

Identification and pathogenicity analysis of Xanthomonas albilineans causing sugarcane leaf scald in Guangxi

作　　者：吴广悦 李奕莎 李美霖 张桂英[1] 陈保善[1] 张木清[1] WU Guangyue;LI Yisha;LI Meilin;ZHANG Guiying;CHEN Baoshan;ZHANG Muqing(Guangxi Key Laboratory of Sugarcane Biology&Provincial and Ministry Jointed Collaborative Innovation Center for Sugar Industry,Guangxi University,Nanning 530005,China)

机构地区：[1]广西甘蔗生物学重点实验室蔗糖产业省部共建协同创新中心,广西大学,南宁530005

出　　处：《植物病理学报》2022年第1期9-16,共8页Acta Phytopathologica Sinica

基　　金：广西科技基地与人才专项(桂科AD20297020);广西大学博士研究生创新项目(YCSW2019038)。

摘　　要：甘蔗白条病是一种检疫性病害,在我国多个蔗区均有报道,严重威胁我国甘蔗生产。本研究对采自广西蔗区不同品种(系)的病原菌进行分离、鉴定、保存,并选取其中40份白条黄单胞菌分离物进行遗传多样性分析与接种试验,评价其致病性。根据三磷酸腺苷结合盒转运蛋白等管家基因和IV型分泌系统virB基因的序列差异和进化树分析,将40个菌株分为两个类型;调查分析接种植株发病症状及病情指数,确定40份病原菌的致病力存在明显差异,致病力强的菌株有5个,中等4个,其他31个菌株表现弱致病力。Sugarcane leaf scald is a quarantine disease that has been reported in many sugarcane planting areas in China.It is a serious threat to sugarcane production in China.This study aimed to isolate,identify,and preserve the pathogen isolates that caused leaf scald disease in different sugarcane varieties in Guangxi.To evaluate their pathogenicity,40 isolates of Xanthomonas albilineans were selected for genetic diversity analysis and pathogenicity assay.According to their sequence differences and phylogenetic tree analysis of housekeeping genes of ATP binding cassette transporter,atpD,gyrB,and virB gene of type IV secretion system,40 isolates were divided into two types.Observation of the symptoms on the inoculated plants and disease index investigation revealed that the pathogenicity among 40 strains was significantly different;of which,5 strains displayed strong pathogenicity,4 strains had medium while 31 strains exhibited weak pathogenicity.

关 键 词：Xanthomonas albilineans 浸渍接种 PCR检测 致病力 

分 类 号：S435.661[农业科学—农业昆虫与害虫防治]