草莓多主棒孢霉小柱孢酮脱水酶基因CcSCD1的功能研究  

Functional analysis of the scytalone dehydratase gene CcSCD1 in Corynespora cassiicola from strawberry

作　　者：薛彩英 武海燕[2] 侯梦圆 马庆周 郭雅双 耿月华[1] 臧睿[1] 张猛[1] 徐超 XUE Caiying;WU Haiyan;HOU Mengyuan;MA Qingzhou;GUO Yashuang;GENG Yuehua;ZANG Rui;ZHANG Meng;XU Chao(College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,China;Analytical Instrument Center,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南农业大学植物保护学院,郑州450002 [2]河南农业大学测试中心,郑州450002

出　　处：《植物病理学报》2022年第1期25-36,共12页Acta Phytopathologica Sinica

基　　金：河南省高校科技创新团队支持计划(18IRTSTHN021)。

摘　　要：多主棒孢霉(Corynespora cassiicola)是世界范围内重要的植物病原真菌,其引起的草莓棒孢霉叶斑病对草莓产业的健康发展具有潜在威胁。小柱孢酮脱水酶(scytalone dehydratase,SCD)是真菌多聚二羟萘类(DHN)黑色素生物合成途径中的关键酶,在植物病原菌致病过程中发挥重要作用。本研究通过同源重组的方法获得了草莓多主棒孢霉小柱孢酮脱水酶基因(CcSCD1)的敲除突变体,并进行了回补和RT-PCR验证。与野生型相比,敲除突变体△CcSCD1-2表现为菌落无色素沉积、菌丝稀疏、产孢量显著下降、分生孢子无色较小以及致病力明显减弱。结果表明,CcSCD1参与调控多主棒孢霉的黑色素生物合成、营养生长、分生孢子产量及致病力。Corynespora cassiicola is a worldwide phytopathogenic fungus.The strawberry leaf spot caused by this pathogen poses a certain potential threat to the healthy development of the strawberry industry.Scytalone dehydratase(SCD)is a key enzyme in the melanin biosynthesis pathway of fungal dihydroxynaphthalene(DHN)melanin,which plays an important role in the pathogenesis of plant pathogens.In this study,the gene-knockout mutants of CcSCD1 were obtained by homologous recombination and verified by RT-PCR.Compared with the wild type,the knockout mutants of CcSCD1 exhibited no pigmentation,sparse hyphae,significantly decreased spore production,colorless conidia and attenuated pathogenicity.These results suggest that CcSCD1 is involved in melanin biosynthesis,vegetative growth,conidiation and pathogenicity of C.cassiicola.

关 键 词：多主棒孢霉 DHN途径 小柱孢酮脱水酶基因 致病力 

分 类 号：S432.44[农业科学—植物病理学]