水稻中MPK5稳态失衡抑制拟禾本科根结线虫与水稻的亲和互作  

Imbalance of MPK5 homeostasis in rice impedes Meloidogyne graminicola and rice susceptible interaction

作　　者：牛永瑞 许立鹤 肖炎农[1] 肖立英 肖雪琼[1] 王高峰[1] NIU Yongrui;XU Lihe;XIAO Yannong;XIAO Liying;XIAO Xueqiong;WANG Gaofeng(The Provincial Key Lab of Plant Pathology of Hubei Province,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学植物科学技术学院,湖北省植物病害监测与安全控制重点实验室,武汉430070

出　　处：《植物病理学报》2022年第1期86-96,共11页Acta Phytopathologica Sinica

基　　金：国家重点研发项目(2017YFD0201604);国家自然科学基金青年基金(31501613);中央高校基本科研业务费专项资金(2662017JC031)。

摘　　要：拟禾本科根结线虫(Meloidogyne graminicola)为水稻的重要土传病原,可严重为害水稻。本研究旨在探究水稻中丝裂原活化蛋白激酶5(MPK5)稳态失衡对拟禾本科根结线虫与水稻亲和互作的影响,并初步探讨其机制。首先,通过线虫侵染试验发现,在水稻中超表达和沉默MPK5引发的MPK5稳态失衡均可抑制拟禾本科根结线虫与水稻的亲和互作。qRT-PCR分析结果显示,与水稻野生型不同,在MPK5超表达和沉默水稻株系中MPK5均受拟禾本科根结线虫诱导表达。同时,拟禾本科根结线虫可抑制MPK5超表达株系中几丁质酶的表达,但诱导MPK5沉默株系中病程相关基因PR5与PR10的表达。上述试验结果表明,MPK5稳态失衡可导致MPK5响应拟禾本科根结线虫的侵染,且在MPK5超表达和沉默水稻株系中MPK5介导水稻产生拟禾本科根结线虫抗性的机制不同。Meloidogyne graminicola is an important soil-borne pathogen of rice that causes significant damage on rice.This study aims to uncover the effect of imbalance of rice mitogen-activated protein kinase gene 5(MPK5)homeostasis on M.graminicola-rice susceptible interaction.Firstly,according to the result of nematode infection test,it was found that the imbalance of MPK5 homeostasis caused by overexpressing and silencing MPK5 in rice led to the inhibition of M.graminicola-rice susceptible interaction.Quantitative RT-PCR assay indicated that MPK5 was up-regulated by M.graminicola infection in both MPK5-overexpressing and silencing rice plants but not in rice wild-type.Meanwhile,upon M.graminicola infection chitinase went down in MPK5-overexpressing rice plant,and the pathogenesis-related genes PR5 and PR10 went up in MPK5-silencing rice plant.These results suggest that the imbalance of MPK5 homeostasis in rice leads to the expression of MPK5 induced by M.graminicola infection,and the imbalance of MPK5 homeostasis results in rice resistance to M.graminicola through different pathways in both MPK5-overexpressing and-silencing rice plants.

关 键 词：拟禾本科根结线虫 水稻 MPK5稳态 亲和互作 

分 类 号：S432.45[农业科学—植物病理学]