DA7R肽修饰的红细胞膜包裹纳米粒的制备与评价  

Preparation and in vitro evaluation of erythrocyte membrane-coated PLGA nanoparticles modified by ^(D)A7R peptides

作　　者：林雯[1] 史琼枝 李银科 曾媛 谢向阳 胡华 LIN Wen;SHI Qiongzhi;LI Yinke;ZENG Yuan;XIE Xiangyang;HU Hua(Department of Clinical Laboratory,Huangshi Love and Health Hospital,Huangshi 435000,China;Department of Pharmacy,General Hospital of Central Theater Command of PLA,Wuhan 430070,China)

机构地区：[1]黄石市爱康医院检验科,黄石435000 [2]中国人民解放军中部战区总医院药剂科,武汉430070

出　　处：《西北药学杂志》2022年第1期71-75,共5页Northwest Pharmaceutical Journal

基　　金：湖北省自然科学基金项目(编号:2020CFB569);湖北省卫生健康科研基金项目(编号:WJ2019H422&WJ2021M218)。

摘　　要：目的制备由^(D)A7R肽修饰的红细胞膜包裹的PLGA纳米粒,并进行体外评价。方法合成DSPE-PEG 2000-^(D)A7R,制备PLGA纳米粒,制备由^(D)A7R肽修饰的红细胞膜包裹纳米粒(^(D)A7R-NP),用透射电子显微镜(transmission electron microscope,TEM)、粒径分析仪等进行表征。结果制备的^(D)A7R-NP平均粒径为(102.8±11.7)nm,包封率为79.14%±2.57%,体外4~8℃可稳定保存30 d,模拟血浆中48 h稳定。细胞实验表明^(D)A7R-NP跨越血脑屏障(blood-brain barrier,BBB)的能力显著提高。结论制备的由^(D)A7R肽修饰的红细胞膜包裹的PLGA纳米粒理化特性良好,且具有良好的脑靶向潜力。Objective To prepare and in vitro evaluate the erythrocyte membrane-coated PLGA nanoparticles modified by ^(D)A7R peptides(^(D)A7R-NP).Methods DSPE-PEG 2000-^(D)A7R was synthesized firstly,then related nanoparticles were prepared,and then ^(D)A7R-NPs were further prepared and their characteristics were assayed by transmission electron microscope(TEM),particle size analyzer and so on.Results The prepared ^(D)A7R-NPs were round and uniform with mean particle size of(102.8±11.7)nm and drug entrapment efficiency of 79.14%±2.57%.^(D)A7R-NPs were stable during the incubation at 4-8℃for 30 d and in simulated plasma for 48 h.Cellular results showed that ^(D)A7R-NPs can efficiently cross blood-brain barrier(BBB)model in vitro.Conclusion The prepared ^(D)A7R-NPs have good physicochemical properties,which can be used as a potential vehicle for the brain targeting drug delivery.

关 键 词：^(D)A7R肽 纳米粒 红细胞膜 脑靶向 

分 类 号：R94[医药卫生—药剂学]