间充质干细胞促进脐带血干细胞体外扩增的研究进展  

Research advances ofumbilical cord blood stem cells proliferation promoted by mesenchymal stem cells in vitro

作　　者：李华 李晓帆[1] 李乃农[1] Li Hua;Li Xiaofan;Li Nainong(Hematopoietic Stem Cell Transplantation Center,Fujian Institute of Hematology,Fujian Provincial Key Laboratory on Hematology,Clinical Research Center for hematological malignancies of Fujian province,Fujian Medical University Union Hospital,Fuzhou 350001,China;Department of Science and Education,The Second Affiliated Hospital of Xiamen Medical college,Xiamen 361021,China)

机构地区：[1]福建医科大学附属协和医院造血干细胞移植中心,福建省血液病研究所,福建省血液病重点实验室,福建省恶性血液病临床医学研究中心,福州350001 [2]厦门医学院附属第二医院科教部,361021

出　　处：《中华细胞与干细胞杂志（电子版）》2022年第1期34-38,共5页Chinese Journal of Cell and Stem Cell（Electronic Edition）

基　　金：国家自然科学基金(81270641,81541024,81200400);厦门市科技局指导性项目(3502Z20179008)。

摘　　要：非亲缘脐带血移植是治疗造血系统疾病的重要移植方式之一,但脐带血移植面临的最大挑战是造血干细胞(HSCs)数量不足,特别是成人患者受到脐带血干细胞数量的限制,导致造血及免疫恢复延迟,非复发死亡率升高。体外扩增脐带血HSCs(UCB-HSCs)是解决该问题的途径之一。研究发现可以通过模拟骨髓造血龛(niche)这一生态位使HSCs在体外进行自我更新增殖,而间充质干细胞(MSCs)正是造血龛的重要的组成细胞之一。本文将探讨MSCs在UCB-HSCs体外扩增中的应用。重点以MSCs促造血的特点、机制,促进脐带血干细胞增殖的各种策略以及其临床应用和前景做一综述。Umbilical cord blood transplantation(UCBT)is one of the most important transplantation in treating hematological disorders.The biggest challenge in UCBT is the insufficient number of hematopoietic stem cells(HSCs),especially in adult patients limited by the number of stem cells from umbilical cord blood,which leads to delayed hematopoietic and immune recovery and increases the risk of infection and early transplant-related death.In vitro expansion of umbilical cord blood HSCs(UCB-HSCs)is one of the ways to solve this problem.Studies have found that HSCs could be self-renewalin vitro by mimetic hematopoietic niches in bone marrow.Mesenchymal stem cells(MSCs)play an important role in niche.In this review,we will discuss the application of MSCs in UCB-HSCs expansion in vitro,focusing on characteristics and mechanisms of hematopoiesis promoted by MSCs,strategies of promoting the proliferation of umbilical cord blood stem cells.Clinical applications and development prospect are also reviewed.

关 键 词：间充质干细胞 脐带血造血干细胞 体外扩增 

分 类 号：R457.7[医药卫生—治疗学]