基于星点设计-效应面法优化红花多糖提取工艺研究  

Optimization of extraction technology of safflower polysaccharide based on central composite design-response surface methodology

作　　者：王锐[1] 李娅兰 白皓天 杨婧[2] WANG Rui;LI Ya-lan;BAI Hao-tian;YANG Jing(School of Pharmacy,Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China;Basic Medical College of Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学药学院,黑龙江哈尔滨150040 [2]黑龙江中医药大学基础医学院,黑龙江哈尔滨150040

出　　处：《海南医学院学报》2022年第5期332-338,共7页Journal of Hainan Medical University

基　　金：国家自然科学基金资助项目(81603418);黑龙江省自然科学基金面上资助项目(H201472);黑龙江优秀青年人才项目(2020YQ05)。

摘　　要：目的:运用星点设计-效应面法探讨红花多糖的最佳提取技术工艺,并对其进行质量评价。方法:以红花多糖的提取率为指标,以料液比、提取时间、提取温度、提取次数为考察因素,在单因素试验的基础上,采用星点设计-效应面法优选出红花多糖的最佳提取工艺并验证。结果:以红花多糖提取率为因变量Y,料液比、提取时间和提取温度为自变量X,P<0.0001建立效应面模型。最佳提取技术工艺为料液比1∶16.69,提取温度91.39℃,提取工作时间89.78 min。在此条件下红花多糖提取率可达7.45%,验证实验结果显示相对标准偏差(RSD)为1.05%,证明该模型能较好地预测实验结果。结论:星点设计-效应面法优化红花多糖的提取率较高,工艺操作简便有效合理、稳定性高、精确度高,可充分发展应用于红花多糖的资源管理开发利用。Objective:To explore the best extraction technology of safflower polysaccharide through central composite design response surface methodology and evaluate its quality.Methods:Taking the extraction rate of Carthamus tinctorius polysaccharide as the index,taking the ratio of solid to liquid,extraction time,extraction temperature and extraction times as the investigation factors,based on the single factor experiment,the central composite design-response surface methodology was used to optimize the optimum extraction process of Carthamus tinctorius polysaccharide and verify the efficacy.Results:The response surface model was established with the extraction rate of Carthamus tinctorius polysaccharide as dependent variable Y,while the ratio of solid to liquid,extraction time and extraction temperature as independent variables X,P<0.0001.The optimum extraction process was as follows:the ratio of solid to liquid was 1∶16.69;the extraction temperature was 91.39℃;the extraction working time was 89.78 min.Under these conditions,the extraction rate of safflower polysaccharide could reach 7.45%.The experimental results showed that relative standard deviation(RSD)was 1.05%and the model could well predict the experimental results.Conclusion:Central composite design-response surface methodology has the advantages of high extraction rate,simple,effective and reasonable process operation,high stability and high precision,which can be fully applied to the resource management and utilization of safflower polysaccharide.

关 键 词：红花多糖 星点设计-效应面法 提取工艺 提取率 

分 类 号：R284.2[医药卫生—中药学]