Orthogonal modular biosynthesis of nanoscale conjugate vaccines for vaccination against infection  

作　　者：Xin Li Chao Pan Peng Sun Zhehui Peng Erling Feng Jun Wu Hengliang Wang Li Zhu 

机构地区：[1]State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Biotechnology,Beijing 100071,China

出　　处：《Nano Research》2022年第2期1645-1653,共9页纳米研究（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.81930122 and U20A20361);the National Key Research and Development Project of China(No.2021YFC2102101).

摘　　要：Conjugate vaccines represent one of the most effective means for controlling the occurrence of bacterial diseases.Although nanotechnology has been greatly applied in the field of vaccines,it is seldom used for conjugate vaccine research because it is very difficult to connect polysaccharides and nanocarriers.In this work,an orthogonal and modular biosynthesis method was used to produce nanoconjugate vaccines using the SpyTag/SpyCatcher system.When SpyTag/SpyCatcher system is combined with protein glycosylation technology,bacterial O-polysaccharide obtained from Shigela flexneri 2a can be conjugated onto the surfaces of different virus-like particles(VLPs)in a biocompatible and controlled manner.After confirming the excellent lymph node targeting and humoral immune activation abilities,these nanoconjugate vaccines further induced efficient prophylactic effects against infection in a mouse model.These results demonstrated that natural polysaccharide antigens can be easily connected to VLPs to prepare highly efficient nanoconjugate vaccines.To the best of the researchers1 knowledge,this is the first time VLP-based nanoconjugate vaccines are produced efficiently,and this strategy could be applied to develop various pathogenic nanoconjugate vaccines.

关 键 词：Shigela flexneri 2a O-POLYSACCHARIDE virus-like particle SpyTag/SpyCatcher system nanoconjugate vaccines 

分 类 号：R392[医药卫生—免疫学]