不同培养液对大菱鲆致病性纤毛虫——贪食迈阿密虫种群增长及复壮毒力的影响  

Effects of different culture media on population growth and virulence rejuvenation of pathogenetic ciliate (Miamiensis avidus) in turbot(Scophthalmus maximus)

作　　者：黄智慧[1,2] 王庆敏 马爱军 郭晓丽[1,2] HUANG Zhihui;WANG Qingmin;MA Aijun;GUO Xiaoli(Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture and Rural Affairs,Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding,Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology,Qingdao 266071,China;Laboratory for Marine Biology and Biotechnology,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266071,China)

机构地区：[1]中国水产科学研究院黄海水产研究所,农业农村部海洋渔业可持续发展重点实验室,山东省海洋渔业生物技术与遗传育种重点实验室,青岛市海水鱼类种子工程与生物技术重点实验室,山东青岛266071 [2]青岛海洋科学与技术试点国家实验室海洋生物学与生物技术功能实验室,山东青岛266071

出　　处：《水产科技情报》2022年第2期95-98,共4页Fisheries Science & Technology Information

基　　金：国家自然科学基金项目(41706168);现代农业产业技术体系专项(CARS-47-G01);山东省良种工程(2019LZGC013);中国水产科学研究院基本科研业务费(2020TD25)。

摘　　要：从山东烟台地区某大菱鲆养殖场的患病大菱鲆体内分离出致病性纤毛虫——贪食迈阿密虫,筛选了4种盾纤毛虫常用体外培养液[大肠杆菌培养液(DH)、牛肉浸膏培养液(BEV)、米粒培养液(RV)、改良L-15培养液(L-15)],研究了不同培养基对纤毛虫种群增长及复壮毒力的影响。试验结果显示:在BEV和L-15两种培养液中,贪食迈阿密虫的种群增长最快,最先达到密度高峰,而DH组和RV组迟24 h才达到种群密度高峰;L-15组纤毛虫种群密度最高,且稳定期长,衰退期晚,RV组次之。结果表明,不同培养液对贪食迈阿密虫的复壮毒力存在不同程度的差异,4种培养液中,改良L-15培养液最符合贪食迈阿密虫的寄生环境,其次是牛肉浸膏培养液。In recent years, studies on the isolation and culture of marine ciliates were limited to the effects of different culture media on population growth, and there were few reports on the toxicological data of pathogenetic ciliates in different culture media.In this experiment, the effects of four culture medias on population growth and virulence rejuvenation of ciliate(Miamiensis avidus) isolated from turbot(Scophthalmus maximus) were studied.The results showed that the density peak of population density was observed earliest in both beef extract culture medium and the modified L-15 group.The density peak in E. coli culture medium and rice grain culture medium was observed 24 hours later.The modified L-15 group had the highest population density with longer stable period and later decay period, and the rice culture medium came next.The virulence rejuvenation test of M. avidus showed that the modified L-15 culture fluid was the most suitable parasitic environment for M. avidus,and the beef extract culture was the second.

关 键 词：大菱鲆 贪食迈阿密虫 纤毛虫 培养液 种群生长 毒力 

分 类 号：S943[农业科学—水产养殖]