馥感啉口服液对卵清蛋白诱发幼龄豚鼠过敏性哮喘的治疗作用  

Therapeutic Effect of Fuganlin Oral Liquid on OVA-induced Allergic Asthma in Juvenile Guinea Pigs

作　　者：郭健敏 李小英 马莉 张文强 陈文培 戴锦龙 江涛 杨威 GUO Jianmin;LI Xiaoying;MALi;ZHANG Wenqiang;CHEN Wenpei;DAI Jinlong;JIANG Tao;YANG Wei(Guangdong Lewwin Pharmaceutical Research Institute Co.,Ltd.,Guangdong Provincial Key Laboratory of Drug Non-Clinical Evaluation and Research,Non-clinical Evaluation Sub-center for Traditional Chinese Medicine of National Engineering Research Center for Modernization of Traditional Chinese Medicine,Guangdong Engineering Research Center for Innovative Drug Evaluation and Research,Guangzhou 510990 Guangdong,China;School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006 Guangdong,China;Guangzhou Yipinhong Pharmaceutical Co.,Ltd,Guangzhou 510760,Guangdong,China)

机构地区：[1]广东莱恩医药研究院有限公司,广东省药物非临床评价研究企业重点实验室,国家中药现代化工程技术研究中心中药非临床评价分中心,广东省创新药物评价与研究工程技术研究中心,广东广州5109901 [2]广东药科大学中药学院,广东广州510006 [3]广州一品红制药有限公司,广东广州510760

出　　处：《中药新药与临床药理》2022年第1期1-6,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家重大新药创制项目(2019ZX09201005-002-006);广东省药物非临床评价研究企业重点实验室项目(2018B030323024);广东省重点领域研发计划“新药创制”重点专项(2019B020202001)。

摘　　要：目的研究馥感啉口服液对卵清蛋白(OVA)诱发幼龄豚鼠过敏性哮喘的治疗作用。方法建立OVA诱导的豚鼠哮喘模型,灌胃给予馥感啉口服液1.79、3.58、7.16、14.31、28.62 g·kg^(-1)(生药量),对照组及模型组给予等量的氯化钠溶液,各阳性对照组分别给予小儿定喘口服液(2.68、5.37、10.74 m L·kg^(-1))和硫酸特布他林(0.28 mg·kg^(-1))。末次激发后采用肺功能震荡仪检测呼吸系统阻力(Respiratory resistance system,Rrs)、呼吸系统弹性(Respiratory system elasticity,Ers)、组织弹性;测定肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中白细胞数、嗜酸性粒细胞数及其比率;ELISA法检测血清中免疫球蛋白(Ig E)水平;肺组织HE染色观察其病理改变。结果馥感啉口服液能降低豚鼠Rrs、Ers和组织弹性,调节呼吸功能;抑制BALF中嗜酸性粒细胞增加;降低血清中Ig E含量;组织病理学显示馥感啉能减轻豚鼠模型肺组织的嗜酸性粒细胞浸润,抑制上皮细胞损伤及脱落,抑制支气管基底膜增生。结论馥感啉口服液可能通过改善呼吸功能、降低气道高反应性、控制气道炎症而发挥抗哮喘作用。Objective To explore the therapeutic effect of Fuganlin oral liquid on ovalbumin(OVA)induced allergic asthma in young guinea pigs.Methods A guinea pig asthma model induced by OVA was established.Five dosages of1.79,3.58,7.16,14.3 and 28.62 g·kg^(-1)crude drug of Fuganlin oral liquid were administered by intragastric administration.Normal saline was administrated in the control group and model group.Commercially available similar drugs were used,three doses of 2.68,5.37 and 10.74 mL·kg^(-1)of pediatric asthma relieving oral solution,and0.28 mg·kg^(-1)of terbutaline sulfate(as positive western medicine).After the final stimulation,a lung function oscillator was used to detect respiratory resistance system,respiratory system elasticity and tissue elastance.Determination of total leukocyte count,eosinophil count and ratio in bronchoalveolar lavage fluid was carried out.Ig E levels in serum were detected with ELISA method.Lung tissues were stained using HE to observed pathological changes.Results Fuganlin oral solution could inhibit guinea pig respiratory resistance system,respiratory system elasticity and tissue elastance,and regulate respiratory function.Fuganlin oral solution could inhibit the increase of eosinophils in BALF,reduce Ig E contents in serum.Histopathological results showed that Fuganlin could alleviate eosinophils infiltration,inhibit the injury and exfoliation of epithelial cells and inhibit the proliferation of bronchial basement membrane in guinea pigs with chronic airway inflammation.Conclusion Fuganlin oral liquid may exert an anti-asthmatic effect by improving respiratory function,reducing airway hyperresponsiveness,and controlling airway inflammation.

关 键 词：馥感啉口服液 过敏性哮喘 呼吸系统阻力 气道高反应性 豚鼠 

分 类 号：R285.5[医药卫生—中药学]