基于UPLC-Q-TOF-MS技术快速分析裂叶独活中香豆素类成分  

Analysis of Chemical Constituents of Coumarins in Heraeleum millefolium by UPLC-Q-TOF-MS

作　　者：顿珠次仁 邓敏芝[2] 朱根华[2] 袁恩[2] 张雪冰婷 严志宏[2] DUNZHU Ciren;DENG Minzhi;ZHU Genhua;YUAN En;ZHANG Xuebingting;YAN Zhihong(Tibetan Traditional Medical College,Lhasa 850000 Tibetan,China;Jiangxi University of Traditional Chinese Medicine,Nanchang 330004 Jiangxi,China)

机构地区：[1]西藏藏医药大学,西藏拉萨850000 [2]江西中医药大学,江西南昌330004

出　　处：《中药新药与临床药理》2022年第1期105-114,共10页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：2018年度西藏自治区藏医药管理局局级科研课题(JJKT2018004)。

摘　　要：目的以超高效液相色谱-四级杆-飞行时间质谱联用(UHPLC-Q-TOF/MS)技术建立裂叶独活中香豆素类化学成分的快速定性分析方法。方法采用Waters Acquity UPLC BEH C_(18)色谱柱(100 mm×2.1 mm,1.7μm);以0.1%甲酸水溶液(A)-乙腈(B)为流动相,梯度洗脱;流速:0.25 mL·min^(-1);柱温:40℃;进样量:2μL。采用电喷雾离子源正离子TOF MS全扫结合IDA-MS2扫描模式收集样品质谱数据。应用PeakView软件中Xic Manager、Formula Finder等模块分析计算,并根据对照品裂解规律、相关文献和各色谱峰的二级碎片离子,鉴定裂叶独活中香豆素类化合物。结果从裂叶独活中共鉴定出34个香豆素类化合物,其中29个为该植物相关文献的首次报道。结论裂叶独活中不同类型香豆素具有独特的裂解规律。所建立的裂叶独活香豆素类化学成分定性分析的方法准确、可靠、高效,适用于香豆素类成分快速鉴定,可为裂叶独活成分及其药效物质基础研究提供参考。Objective To establish a rapid qualitative analysis method for coumarin in Heraeleum millefolium by using UPLC-Q-TOF-MS. Methods The separation was performed on Waters Acquity UPLC BEH C18(100 mm ×2.1 mm, 1.7 μm) column with gradient elution of 0.1% formic acid(A)-acetonitrile(B), The flow rate was0.25 mL·min;,the column temperature was 40℃,and the injection quantity was 2 μL. ESI positive ion mode was used for acquisition and detection,and the MS and MS/MS data were collected by full scanning and information dependent acquisition(IDA)mode,respectively. The coumarin compounds in H. millefolium were identified by using PeakView software,combined with the fragmentation behaviors of standard compounds,related references,and the fragment ions of each chromatographic peak. Results A total of 34 coumarin compounds in H. millefolium were identified,among them,29 coumarins were reported from this plant for the first time. Conclusion Different types of coumarins in H. millefolium showed unique fragmentation patterns. The method of qualitative analysis of coumarins in H. millefolium established in this study is accurate,reliable and efficient,which is suitable for rapid identification of coumarins, and provides an experimental basis for the research on the ingredients of H. millefolium and the subsequent elucidation of its medicinal material basis.

关 键 词：裂叶独活 超高效液相色谱-四级杆-飞行时间质谱联用 香豆素 化学成分 

分 类 号：R284.1[医药卫生—中药学]