Development of a Recombinase-aided Amplification Combined With Lateral Flow Dipstick Assay for the Rapid Detection of the African Swine Fever Virus  

作　　者：LI Jiang Shuai HAO Yan Zhe HOU Mei Ling ZHANG Xuan ZHANG Xiao Guang CAO Yu Xi LI Jin Ming MA Jing ZHOU Zhi Xiang 

机构地区：[1]Faculty of environment and life,Beijing University of Technology,Beijing 100024,China [2]State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China [3]National Institute of Viral Disease Control and Prevention,Beijing 100052,China [4]China Animal Health and Epidemiology Center,Qingdao 266032,Shandong,China

出　　处：《Biomedical and Environmental Sciences》2022年第2期133-140,共8页生物医学与环境科学（英文版）

基　　金：supported by National Key R&D Program of China[2017YFC200503];National Natural Science Foundation of China[No.42077399].

摘　　要：Objective To establish a sensitive,simple and rapid detection method for African swine fever virus(ASFV)B646L gene.Methods A recombinase-aided amplification-lateral flow dipstick(RAA-LFD)assay was developed in this study.Recombinase-aided amplification(RAA)is used to amplify template DNA,and lateral flow dipstick(LFD)is used to interpret the results after the amplification is completed.The lower limits of detection and specificity of the RAA assay were verified using recombinant plasmid and pathogenic nucleic acid.In addition,30 clinical samples were tested to evaluate the performance of the RAA assay.Results The RAA-LFD assay was completed within 15 min at 37°C,including 10 min for nucleic acid amplification and 5 minutes for LFD reading results.The detection limit of this assay was found to be 200 copies per reaction.And there was no cross-reactivity with other swine viruses.Conclusion A highly sensitive,specific,and simple RAA-LFD method was developed for the rapid detection of the ASFV.

关 键 词：African swine fever virus Recombinase aided amplification Lateral flow detection 

分 类 号：S852.65[农业科学—基础兽医学]