细胞钙离子荧光探针研究进展  

Research progress of cellular Ca;fluorescent probes

作　　者：黄致远 赵旭鹏 徐伟刚 张坤 HUANG Zhiyuan;ZHAO Xupeng;XU Weigang;ZHANG Kun(School of Basic Medicines,Naval Medical University,Shanghai 200433,China;Department of Diving and Hyperbaric Medicine,Naval Special Medicine Center,Naval Medical University,Shanghai 200433,China)

机构地区：[1]海军军医大学基础医学院,上海200433 [2]海军军医大学海军特色医学中心潜水与高气压医学研究室,上海200433

出　　处：《生命的化学》2021年第12期2598-2605,共8页Chemistry of Life

基　　金：国家自然科学基金项目(81801866)。

摘　　要：钙离子(Ca;)广泛参与细胞生理病理反应,其含量和在胞内位置的改变可激发不同效应。因此,快速有效检测胞内Ca;浓度及其分布对于了解其在细胞活动中的作用具有重要意义。荧光探针法由于灵敏度高、使用方便、成本低廉等优点得到广泛应用,但不同类型的探针在准确性、灵敏度和稳定性等方面各有优劣。本文系统总结了现有的Ca;化学荧光探针和基因编码探针,为开展Ca;相关研究时合理选择检测方法提供参考。Calcium ion(Ca;) is widely involved in physiological and pathological processes in cells, and different effects can be produced following its changes. Therefore, varies fluorescent probes have been widely used for detecting Ca;concentration and distribution. However, all the probes have their own advantages and disadvantages in terms of accuracy, sensitivity and stability. This paper systematically summarizes the existing Ca;chemical fluorescent probes and genetically encoded fluorescent probes.

关 键 词：荧光探针 钙离子 化学荧光探针 基因编码探针 

分 类 号：Q257[生物学—细胞生物学]