Dysbindin-1与脆性X相关蛋白1、Y盒结合蛋白2的相互作用  

Verification of the interaction between dysbindin-1 with FXR1P and YBX2

作　　者：李海雁 张姝 张盼盼[1] 孙欣[1] 袁业锋 冯雅琴[1] LI Haiyan;ZHANG Shu;ZHANG Panpan;SUN Xin;YUAN Yefeng;FENG Yaqin(Department of Histology and Embryology,Shanxi Medical University,Taiyuan 030001,China;Beijing Children's Hospital,Capital Medical University,Beijing 100045,China)

机构地区：[1]山西医科大学组织胚胎学教研室,太原030001 [2]首都医科大学附属北京儿童医院,北京100045

出　　处：《生命的化学》2021年第12期2667-2675,共9页Chemistry of Life

基　　金：国家自然科学基金项目(30900777);山西省青年科技研究基金项目(2010021036-1);山西医科大学博士启动基金项目(03200804)。

摘　　要：(肌)营养不良短小蛋白结合蛋白1(dystrobrevin-binding protein 1, dysbindin-1)是溶酶体相关细胞器生物发生复合体-1(biogenesis of lysosome-related organelles complex-1, BLOC-1)的一个亚基,在内体-溶酶体通路的货物运输中发挥一定作用。Dysbindin-1在睾丸组织中的作用尚不明确。本文从前期已筛选到的dysbindin-1在小鼠睾丸组织中的潜在互作蛋白质中,选取2个RNA结合蛋白(RNA-binding proteins, RBPs)脆性X相关蛋白1(fragile X related protein 1, FXR1P)和Y盒结合蛋白2(Y-box binding protein 2, YBX2),用GST pull-down和内源性免疫沉淀的方法进行相互作用的验证。结果显示,dysbindin-1与FXR1P和YBX2存在相互作用,提示dysbindin-1在睾丸组织RNA的运输中可能发挥作用。Dystrobrevin-binding protein 1(dysbindin-1), a subunit of biogenesis of lysosome-related organelles complex-1(BLOC-1), participates in the cargos trafficking in the endosome-lysosome transport.Function of dysbindin-1 is uncertain in the testis. RNA-binding proteins of fragile X related protein 1(FXR1P)and Y-box binding protein 2(YBX2) are candidates for dysbindin-1 interaction proteins in the mouse testis.GST pull-down assay and native immunoprecipitation(IP) were performed to verify the interaction between dysbindin-1 with FXR1P and YBX2. The results show that dysindin-1 interacts with FXR1P and YBX2,respectively, which suggests dysbindin-1 may play a role in RNAs transportation in testis tissue.

关 键 词：dysbindin-1 FXR1P YBX2 相互作用 小鼠 睾丸 

分 类 号：R346[医药卫生—基础医学]