分子荧光光谱法测定富硒大米中的痕量硒  

Determination of Trace Selenium in Selenium-enriched Rice by Molecular Fluorescence Spectrometry

作　　者：罗建民 彭翠红 杨智威 LUO Jianmin;PENG Cuihong;YANG Zhiwei(School of Chemistry and Civil Engineering,Shaoguan University,Shaoguan,Guangdong 512005,China)

机构地区：[1]韶关学院化学与土木工程学院,广东韶关512005

出　　处：《中国无机分析化学》2022年第2期81-85,共5页Chinese Journal of Inorganic Analytical Chemistry

基　　金：广东省自然科学基金面上资助项目(2021A1515010060);广东省科技创新战略专项项目(韶科〔2020〕44号);广东省普通高校科研项目(2019KTSCX162);韶关市科技计划项目(2019sn055)。

摘　　要：硒(Ⅳ)与DAN(2,3-二氨基萘)生成具有荧光特性的4,5-苯并苤硒脑,可用分子荧光光谱法测定,优化测定的实验条件,建立了快速准确测定大米中痕量硒的方法。结果表明,其最佳测定条件为:pH=2.0,反应温度为80℃,1.0×10^(-5) mol/L十六烷基溴化吡啶用作表面活性剂,环己烷萃取条件下,增敏效果明显,在0~0.067μg/mL浓度范围内线性关系良好,方法检出限为6.5×10^(-4)μg/mL,并且将方法应用于三个不同地区的大米中硒含量的测定,相对标准偏差为1.9%~2.8%,加标回收率在87.8%~107%,表明方法准确可靠。Trace Se can be determined by molecular fluorescence spectrophotometry when selenium(Ⅳ)with DAN(2,3-diaminonaphthalene)was carried out to produce 4,5-benzoselenol with strong fluorescence characteristics.The conditions for the determination of trace Se were optimized,and the fast determination of trace Se in selenium-enriched rice were established.The results showed that the optimal test conditions were as the follow:pH=2.0,80℃,1.0×10^(-5) mol/L CPB in cyclohexane,the sensitization effect was obvious.Under the optimum conditions,a good linearity was observed in the range of 0—0.067μg/mL with the detection limit is 6.5×10^(-4)μg/mL.The proposed method was applied to the determination of the trace selenium in selenium-enriched rice from three different provinces,the RSD of the determination results were 1.9%—2.8%.The recoveries of samples were 87.8%—107%,the result showed the method is reliable and sensitive.

关 键 词：硒 分子荧光光谱法 富硒大米 十六烷基溴化吡啶 

分 类 号：O657.3[理学—分析化学] TH441.6[理学—化学]