刺五加浸膏对手机辐射剥夺睡眠果蝇的影响  

Effects of Acanthopanax Senticosus Extract on Drosophila Melanogaster Sleep Deprivation by Mobile Phone Radiation

作　　者：卞宏生[1] 岳骊郦 沙吾列西·木拉提别克 毛登烜 郭红丽 黄莉莉[1] BIAN Hongsheng;YUE Lili;SHAWULIEXI Mulatibieke;MAO Dengxuan;GUO Hongli;HUANG Lili(College of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学院药学院,哈尔滨150040

出　　处：《医药导报》2022年第2期155-161,共7页Herald of Medicine

基　　金：国家自然科学基金资助项目(82003974);国家自然科学青年科学基金资助项目(81603282);黑龙江省自然科学基金联合引导项目(LH2019H106);教育部重点实验室—北药基础与应用研究开放课题基金资助;黑龙江中医药大学校科研基金资助黑龙江中医药大学博士创新基金(2015bs08);2019年黑龙江省大学生创新创业训练计划项目(201910228036)。

摘　　要：目的观察刺五加浸膏对手机辐射剥夺果蝇睡眠的改善作用。方法将果蝇随机分为空白组,模型组,低、中、高浓度给药组,每组32只。利用果蝇活动监测系统(DAMS),以平均睡眠时间和睡眠-觉醒节律为观察指标,空白对照组和模型对照组分别选择7日龄果蝇检测24 h,其中模型对照组果蝇在检测时给予手机辐射影响,辐射量:30~190μW·(cm^(2))^(-1),干预点:20:00—22:00,每30 min拨打电话10 min。低、中、高浓度给药组于果蝇4日龄分别给予2%,4%,8%刺五加浸膏配置培养基给药3 d,并于7日龄放入已给药检测管中检测24 h,并给予模型对照组相同条件的手机辐射干预。结果与空白对照组比较,模型对照组果蝇因夜晚手机辐射干预,雌雄果蝇睡眠、觉醒均出现交替性变化现象(片段化睡眠),其夜晚12 h睡眠时间均缩短(P<0.01)。与模型对照组比较,给药组刺五加能通过减少辐射干预期间果蝇的片段化睡眠,增加睡眠时长,改善果蝇的睡眠-觉醒节律状态。雄果蝇2%给药剂量白天、夜晚、全天睡眠时间明显延长(P<0.01),8%给药剂量白天、全天睡眠时间明显延长(P<0.01或P<0.05)。其中2%给药剂量的睡眠-觉醒节律变化最为明显,与空白对照组节律分布最接近;雌果蝇4%给药剂量白天睡眠时间明显缩短,8%给药剂量夜晚睡眠时间明显延长(P<0.01)。其中8%给药剂量睡眠-觉醒节律变化最为明显,与空白对照组节律分布也最接近。结论刺五加浸膏对手机辐射条件下果蝇的睡眠时间和睡眠-觉醒节律的负性影响具有正向干预作用。Objective To observe the effect of Acanthopanax Senticosus extract on the sleep deprivation of Drosophila melanogaster by mobile phone radiation.Methods The experiment was randomly divided into five groups:blank control group,model control group,and low-,medium-,and high dose group,with 32 Drosophila melanogaster in each group.The average sleep time and sleep-wake rhythm were used as the observation indexes by using the DAMS.The seven-day-old Drosophila melanogaster were tested for 24 hours in the blank control group and the model control group,and the Drosophila melanogaster were exposed to mobile phone radiation[30-190μW·(cm^(2))^(-1),intervention point:20:00-22:00 every 30 minutes to make phone call for 10 minutes]in the model control group.The low-,medium-and high dose group were treated with different concentrations(2%,4%,and 8%)of Acanthopanax Senticosus extract for three days with the four-year-old,and also irradiated by mobile phone for 24 hours at the age of seven days.Results Compared with the blank control group,Drosophila melanogaster in the model control group which was intervened in the night by mobile phone radiation showed an alternate change on sleeping and awakening(fragmentation sleep),and the sleep duration of 12 hours was shortened(P<0.01).This phenomenon indicates that mobile phone radiation has a negative effect on Drosophila’s sleep.Compared with the model control group,Acanthopanax Senticosus could improve the sleep-wake rhythm of Drosophila melanogaster by reducing the fragmented sleep and increasing the sleep duration.The results showed that the daytime,night and all-day sleep time of male Drosophila melanogaster at 2%dosage were significantly prolonged(P<0.01),and the daytime and all-day sleep time of 8%dosage were significantly prolonged(P<0.01 or P<0.05).The sleep-wake rhythm of 2%dosed drosophila melanogaster was the most obvious and similar to that of the blank control group,and the sleep time of 4%dosed Drosophila melanogaster was obviously shortened during the day,and the sle

关 键 词：刺五加浸膏 手机辐射 果蝇 睡眠-觉醒节律 果蝇活动检测系统 

分 类 号：R965[医药卫生—药理学]