藏药七味兔耳草散的质量控制  

Quality Control of Tibetan Qiwei Tuercao San

作　　者：刘安平 卜晨琛 马洁琼 苏媛 李维业 马秀玲 任东海 杨增亮 LIU Anping;BU Chenchen;MA Jieqiong;SU Yuan;LI Weiye;MA Xiuling;REN Donghai;YANG Zengliang(Xining Center for Food and Drug Inspection&Testing,Xining 810000,China;Qinghai Provincial Drug Inspection and Testing Institute,Xining 810000,China;Qinghai Hospital of Traditional Chinese Medicine,Xining 810000,China;Golmud Drug Administration Service Center,Qinghai Province,Golmud 816099,China)

机构地区：[1]西宁市食品药品检验检测中心,西宁810000 [2]青海省药品检验检测院,西宁810000 [3]青海省中医院,西宁810000 [4]青海省格尔木市药品管理服务中心,格尔木816099

出　　处：《医药导报》2022年第2期239-243,共5页Herald of Medicine

基　　金：青海省科技计划项目(2020-ZJ-747)。

摘　　要：目的完善藏药七味兔耳草散质量标准。方法采用显微定性特征鉴别朱砂、姜黄、红花、手参;建立短穗兔耳草、诃子、红花、熊胆粉、姜黄的薄层色谱(TLC)定性鉴别;采用高效液相色谱(HPLC)法测定没食子酸和木犀草素的含量。结果新建立的显微鉴别与TLC鉴别特征明显,可行性良好;没食子酸在0.002023~0.02023μg的范围内线性关系良好(r=0.9996),木犀草素在0.197~1.97μg的范围内线性关系良好(r=0.9996)。结论该文建立分析方法科学、准确、可靠;进一步提高该制剂的质量标准,能够更好、更全面地控制七味兔耳草散的质量。Objective To improve the quality standard of Tibetan Qiwei Tuercao San.Methods Microscopic identification of Cinnabaris,Curcumae longae rhizoma,Carthami flos,and Gymnadenia R.Br were set up.TLC was set up for the qualitative identification of Lagotis brachystachya Maxim,Chebulae fructus,Carthami flos,Bear bile powder and Curcumae longae rhizoma.An HPLC method was used to determine the contents of gallic acid and luteolin.Results The new established microscopic identification and TLC identification have obvious characteristics resolution and good feasibility.Gallic acid in the range of 0.002023-0.02023μg showed good linear relationship(r=0.9996),and luteolin in the range of 0.197-1.97μg showed good linear relationship(r=0.9996).Conclusion The new analysis methods established in this study was scientific,accurate and reliable.It can further improving the quality standard of the preparation and can better control the quality of Tibetan Qiwei Tuercao San.

关 键 词：七味兔耳草散 诃子 短穗兔耳草 熊胆粉 朱砂 姜黄 红花 手参 没食子酸 木犀草素 

分 类 号：R927.2[医药卫生—药学] R29