泛耐药鲍曼不动杆菌生物被膜形成能力与同源性分析  

Analysis of Biofilm Formation Ability and Homology of Extensively Drug-resistant Acinetobacter baumannii

作　　者：孟千琳 凌保东[1,2] MENG Qianlin;LING Baodong(Key Laboratory of Sichuan Province College for Structural Specific Small Molecule Drug Research,Chengdu 610500,China;School of Pharmacy,Chengdu Medical College,Chengdu 610500,China)

机构地区：[1]成都医学院结构特异性小分子药物研究四川省高校重点实验室,成都610500 [2]成都医学院药学院,成都610500

出　　处：《医药导报》2022年第2期244-247,共4页Herald of Medicine

基　　金：国家自然科学基金资助项目(81373454)。

摘　　要：目的研究泛耐药鲍曼不动杆菌(XDRAB)的同源性及其生物被膜的形成能力,为临床防控感染提供依据。方法收集成都医学院第一附属医院2018年1月—2020年7月XDRAB 37株,采用微量肉汤稀释法测定16种抗菌药物对37株XDRAB的最低抑菌浓度(MIC),结晶紫染色法检测生物被膜的形成能力,运用多位点序列分型方法(MLST)和肠杆细菌基因间重复共有序列聚合酶链反应(ERIC-PCR)对37株XDRAB进行分型检测。结果 37株XDRAB仅对多粘菌素类、替加环素敏感,其余抗菌药物均耐药。生物被膜形成能力与阴性对照组比较,81%以上XDRAB均形成生物被膜。MLST分型结果:37株XDRAB均属于国际克隆组IC2的克隆复合体(CC92),其中34株为ST208,其次为ST195和ST368。ERIC-PCR分型结果:37株XDRAB分为两种类型,其中Ⅰ型22株,Ⅱ型15株。结论临床分离的XDRAB生物被膜形成能力强,CC92为XDRAB院内感染流行的主要克隆复合体,MLST与ERIC-PCR可作为XDRAB同源性分析的有效方法。Objective To research the homology of Extensively Drug-resistant Acinetobacter baumanii(XDRAB) and its biofilm formation ability,which will provide a basis for the clinical infection prevention and control.Methods Thirty-seven XDRAB isolates were collected from January 2018 to July 2020,which from the First Affiliated Hospital of Chengdu Medical College.The minimum inhibitory concentration(MIC) of 16 antibacterial agents against 37 strains of XDRAB were determined by the microdilution broth method,and the crystal violet staining method was used to quantitatively analyze their biofilm formation ability.The multilocus sequence typing(MLST) and the enterobacterial intergenic repeat consensus sequence PCR(ERIC-PCR) were used to test 37 XDRAB strains.Results The 37 strains of XDRAB were only sensitive to polymyxins and tigecycline,but were resistant to the other antibacterial agents.Compared with the negative control group,the biofilm formation ability of XDRAB was more than 81%.For the results of MLST typing,37 strains of XDRAB belonged to the international cloning group IC2 cloning complex(CC92),of which 34 strains were ST208,followed by ST195 and ST368.ERIC-PCR typing results showed that 37 strains of XDRAB were divided into two types,of which type I contained 22 strains and type II contained 15 strains.Conclusion The clinically isolated XDRAB biofilm formation ability was strong.CC92 was the main clonal complex of XDRAB nosocomial infection.MLST and ERIC-PCR can be used as effective methods for XDRAB homology analysis.

关 键 词：泛耐药鲍曼不动杆菌 生物被膜 同源性分析 

分 类 号：R978[医药卫生—药品] R378[医药卫生—药学]