姜黄素类似物L50H8对卵巢癌OVCAR3细胞增殖、侵袭与凋亡的影响及其机制研究  

Effects of Curcumin Analogue L50H8 on Proliferation,Invasion and Apoptosis of Ovarian Cancer OVCAR3 Cells and Its Mechanism

作　　者：邱扬 陈龙飞 王晓玉[3] 林绍强[4] QIU Yang;CHEN Longfei;WANG Xiaoyu;LIN Shaoqiang

机构地区：[1]江门市五邑中医院妇科,广东江门529000 [2]佛山市第一人民医院妇科,广东佛山528010 [3]暨南大学附属第一医院妇科,广东广州510630 [4]暨南大学附属第一医院中心实验室,广东广州510630

出　　处：《新中医》2022年第1期134-140,共7页New Chinese Medicine

摘　　要：目的:研究姜黄素类似物L50H8对卵巢癌OVCAR3细胞增殖、侵袭与凋亡的影响,并探讨其作用机制。方法:MTT法检测L50H8对细胞增殖的影响;Annexin V/PI双染色流式细胞法检测L50H8对细胞凋亡的影响;Transwell法检测L50H8对细胞侵袭力的影响;Western blot法探讨L50H8影响细胞侵袭的机制:检测其对细胞侵袭相关因子细胞外基质金属蛋白酶诱导因子(CD147)、基质金属蛋白酶(MMP)2、MMP9和血管内皮生长因子(VEGF)表达的影响;探讨其诱导细胞凋亡的机制:检测其对内质网应激(ERS)启动因子葡萄糖调节蛋白78(GRP78)及前凋亡因子C/EBP-同源蛋白(CHOP)表达的影响。结果:L50H8能显著抑制OVCAR3细胞增殖,24 h的IC_(50)为(3.08±0.08)μmol/L。L50H8能诱导OVCAR3细胞凋亡,其凋亡诱导效应强于姜黄素。Western blot法发现L50H8能下调CD147、MMP2、MMP9及VEGF的表达,上调GRP78及CHOP的表达。结论:L50H8能显著抑制OVCAR3细胞增殖,通过下调CD147、MMP2、MMP9及VEGF降低细胞侵袭力,引发ERS诱导细胞凋亡。Objective:To study the effects of curcumin analogue L50 H8 on proliferation,invasion and apoptosis of ovarian cancer OVCAR3 cells and explore its mechanism.Methods:The effect of L50 H8 on cell proliferation was detected by MTT assay;Annexin V/PI double staining flow cytometry was used to detect the effect of L50 H8 on apoptosis.The effect of L50 H8 on cell invasion was detected by Transwell method.To explore the mechanism of L50 H8 affecting cell invasion by Western blot.To detect its effect on the expression of extracellular matrix metalloproteinase inducer(CD147),matrix metalloproteinase 2(MMP2),MMP9 and vascular endothelial growth factor(VEGF).To explore the mechanism of L50 H8 inducing cell apoptosis,its effects on the expression of endoplasmic reticulum stress(ERS)promoter glucose regulated protein 78(GRP78)and preapoptotic factor C/EBP-homologous protein(CHOP)were detected.Results:L50 H8 could significantly inhibit the proliferation of OVCAR3 cells,and the IC_(50) at 24 h was(3.08±0.08)μmol/L.L50 H8 can induce apoptosis of OVCAR3 cells,and its apoptosis inducing effect is stronger than curcumin.Western blot showed that L50 H8 can down regulate the expression of CD147,MMP2,MMP9 and VEGF,and up regulate the expression of GRP78 and CHOP.Conclusion:L50 H8 can significantly inhibit the proliferation of OVCAR3 cells,reduce cell invasion by down regulating CD147,MMP2,MMP9 and VEGF,and induce ERS induced apoptosis.

关 键 词：姜黄素类似物 L50H8 卵巢癌 OVCAR3细胞 细胞侵袭 细胞凋亡 内质网应激 

分 类 号：R285.5[医药卫生—中药学]