吲哚美辛治疗口腔溃疡的动物实验研究  

Animal Experimental Study of Indomethacin in The Treatment of Oral Ulcer

作　　者：张少璐 骆树瑜[3] 邱玉玲[1] 张向宇[3] 钟玉绪 孔德新[1] ZHANG Shao-Lu;LUO Shu-Yu;QIU Yu-Ling;ZHANG Xiang-Yu;ZHONG Yu-Xu;KONG De-Xin(School of Pharmacy,Tianjin Medical University,Tianjin 300070,China;Stomatology Hospital,Tianjin Medical University,Tianjin 300070,China;State Key Laboratory of Toxicology and Medical Countermeasures,Beijing Institute of Pharmacology and Toxicology,Beijing 100089,China)

机构地区：[1]天津医科大学药学院,天津300070 [2]军事医学研究院毒物药物研究所,天津300070 [3]天津医科大学口腔医院,北京100089

出　　处：《生物化学与生物物理进展》2022年第2期413-419,共7页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金(82073890,81673464);天津市教委科研计划(2020KJ186)资助项目。

摘　　要：目的探讨两种吲哚美辛制剂在口腔溃疡愈合中的作用。方法大鼠右颊用铁钉烫烧造模,分为吲哚美辛凝胶给药组(YN)、复方苯佐卡因凝胶阳性对照组(NP)、吲哚美辛喷雾给药组(YP)、口腔炎喷雾阳性对照组(PP)、正常对照组(N)和阴性对照组(M)。采用HE染色、组织学标准定量评价溃疡面再上皮化及肉芽组织形成情况,比色法检测溃疡组织一氧化氮(nitric oxide,NO)、一氧化氮合酶(nitric oxide synthase,NOS)活性水平,免疫组化检测大鼠溃疡组织表皮生长因子(EGF)及其受体(EGFR)、血管内皮细胞生长因子(VEGF)表达情况。结果YP、YN、NP、PP组溃疡面积较M组均减小,组织学定量评价显示YP、YN、NP、PP组得分均高于M组(P<0.05);给药后,YP组NO和NOS水平显著低于M组(P<0.05);EGF在YP、YN、NP、PP组的表达均高于M组和N组(P<0.05);EGFR在YP、YN、NP、PP组的表达与N组相比明显升高(P<0.05);VEGF在YP、YN、NP、PP组的表达均低于M组(P<0.05)。结论两种吲哚美辛制剂均能起到减轻大鼠口腔溃疡炎症反应、促进愈合作用。Objective To investigate the effect of two indomethacin preparations on the healing process of oral ulcers. Methods After the right cheek of rats were burned with nails,they were divided into indomethacin gel to medicine group(YN), compound benzocaine gel positive control group(NP), indomethacin spray to medicine group(YP), stomatitis spray, normal control group(PP), positive control group(N) and the negative control group(M). The reepithelialization of ulcer surface and granulation tissue formation was evaluated after HE staining with histological quantitative standard evaluation. The level of nitric oxide(NO) and nitric oxide synthase(NOS) in ulcer tissues was detected by colorimetry. The expressions of epidermal growth factor(EGF), as well as its receptor(EGFR) and vascular endothelial cell growth factor(VEGF) in rat ulcer tissues, were detected by immunohistochemistry. Results The ulcer area of the YP, YN, NP, and PP groups was smaller than that of the M group. The quantitative histological evaluation showed that the scores of the YP, YN, NP, and PP groups were higher than that of the M group(P<0.05). After administration, the level of NO and NOS in the YP group were significantly lower than that of M group(P<0.05). The EGF expression of YP, YN, NP, and PP groups was higher than that of the M and N groups(P<0.05). EGFR was expressed in the epithelium from the cytoplasm to the membrane, and the expression level in the YP, YN, NP, and PP was significantly higher than that of the N group(P<0.05). The VEGF expression of YP, YN, NP, and PP groups was lower than that of the M group(P<0.05).Conclusion Both two indomethacin preparations can reduce the inflammatory response and promote the healing of oral ulcers in rats.

关 键 词：口腔溃疡 吲哚美辛 大鼠模型 愈合 

分 类 号：R9[医药卫生—药学] R78