阿尔茨海默病及轻度认知功能障碍患者单核细胞的Aβ42吞噬功能研究  

Aβ42 phagocytosis of monocytes in patients with Alzheimer’s disease and mild cognitive impairment

作　　者：梁庆华[1] 利荣乔 何本进[1] LIANG Qinghua;LI Rongqiao;HE Benjin(Jiangbin Hospital,Guangxi Zhuang Autonomous Region,Nanning 530021,China)

机构地区：[1]广西壮族自治区江滨医院,南宁530021

出　　处：《中国免疫学杂志》2022年第4期474-477,共4页Chinese Journal of Immunology

摘　　要：目的:探讨阿尔茨海默病(AD)及轻度认知功能障碍(MCI)患者血液中单核细胞的β淀粉样蛋白42(Aβ42)吞噬功能研究,为AD的诊治提供新的方向。方法:选择2016年12月至2019年12月广西壮族自治区江滨医院就诊的AD患者(AD组)、MCI患者(MCI组)和体检健康志愿者(NC组)各56例,采用流式细胞术分析外周血中单核细胞(CD14^(+)CD16^(-)、CD14^(+)CD16^(+)和CD14^(−)CD16^(+))的Aβ42吞噬功能,比较不同年龄组[20~45岁(低龄组)、46~65岁(正常组)、和66~80岁(高龄组)]AD患者外周血单核细胞的Aβ42吞噬功能变化;ELISA检测血液和脑脊液中Aβ42水平;Pearson相关分析检测外周血单核细胞的Aβ42吞噬功能与血液和脑脊液Aβ42水平的相关性。结果:与NC组和MCI组相比,AD患者总单核细胞和单核细胞亚群(CD14^(+)CD16^(-)、CD14^(+)CD16^(+)和CD14^(−)CD16^(+))的Aβ42吞噬功能显著降低(P<0.05),MCI组和NC组间差异无统计学意义;AD高年龄组患者外周血总单核细胞和单核细胞亚群(CD14^(+)CD16^(-)、CD14^(+)CD16^(+)和CD14^(−)CD16^(+))的Aβ42吞噬功能显著降低(P均<0.05);与NC组和MCI组相比,AD患者血液和脑脊液中Aβ42水平显著降低(P<0.05),MCI组与NC组相比下降不显著;外周血中单核细胞CD14^(+)CD16^(-)、CD14^(+)CD16^(+)亚群与血液Aβ42水平呈正相关(r=0.412,P=0.012;r=0.356,P=0.032)。结论:AD患者外周血单核细胞Aβ42吞噬功能下降,提示其在AD的发生发展中发挥重要作用,为AD的诊治提供了潜在靶点。Objective:To investigate the phagocytic function ofβ-amyloid 42(Aβ42)in blood monocytes of Alzheimer's disease(AD)and mild cognitive impairment(MCI)patients,and to provide a new therapeutic direction for the diagnosis and treat⁃ment of AD.Methods:56 cases of AD patients(AD group),MCI patients(MCI group)and healthy subjects(NC group)admitted to Jiangbin Hospital of Guangxi Zhuang Autonomous Region from December 2016 to December 2019 were selected;analysis of peripheral blood mononuclear cells(CD14^(+)CD16^(-),CD14^(+)CD16^(+) and CD14^(−)CD16^(+))Aβ42 phagocytosis by using flow cytometry;comparing different age groups[20~45 year old(young group),46~65 year old(normal group),and 66~80 year old(older group)]of peripheral blood mononuclear cells Aβ42 phagocytosis changes in patients with AD;Aβ42 level in blood and cerebrospinal fluid were detected by ELISA;correlation between phagocytosis of Aβ42 in peripheral blood mononuclear cell and Aβ42 level in blood and cerebrospinal fluid was analyzed by Pearson correlation.Results:Compared with NC and MCI groups,phagocytosis of Aβ42 in total monocytes and monocyte subsets(CD14^(+)CD16^(-),CD14^(+)CD16^(+) and CD14^(−)CD16^(+))in AD patients were significantly decreased(P<0.05),but there was no significant difference between MCI group and NC group;phagocytosis of Aβ42 in total monocytes and monocyte subsets(CD14^(+)CD16^(-),CD14^(+)CD16^(+)and CD14^(−)CD16^(+))in peripheral blood of patients with AD in high age group were significantly decreased(all P<0.05);compared with NC and MCI groups,levels of Aβ42 in blood and cerebrospinal fluid in AD patients were significantly decreased(P<0.05),but not significantly decreased in MCI group compared with NC group.CD14^(+)CD16^(-) and CD14^(+)CD16^(+) subsets in peripheral blood were positively correlated with blood Aβ42 level(r=0.412,P=0.012;r=0.356,P=0.032).Conclusion:The decreased phagocytosis of Aβ42 in peripheral blood of AD patients suggests that Aβ42 plays an important role in occurrence and development of AD

关 键 词：阿尔茨海默病 单核细胞亚群 Β淀粉样蛋白 吞噬功能 

分 类 号：R446.6[医药卫生—诊断学]