HIV抗体确证试验条带用于新发感染判定的研究  

Study on the application of HIV antibody confirmatory test strip results to the determination of new infections

作　　者：边仕超[1] 余刚[1] 吉克春农 王菊[1] 肖琳[1] 谌茂刚 陈晓茹 洪静 BIAN Shichao;YU Gang;JIKE Chunnong;WANG Ju;XIAO Lin;CHEN Maogang;CHEN Xiaoru;HONG Jing(Liangshan Center for Disease Control and Prevention,Xichang 615000,Sichuan,China)

机构地区：[1]凉山州疾病预防控制中心,四川西昌615000

出　　处：《中国艾滋病性病》2022年第2期191-194,共4页Chinese Journal of Aids & STD

摘　　要：目的分析凉山州HIV-1新发感染检测结果与四种确证试剂检测带型相关性,了解新近感染者与长期感染者在四种确证检测试剂中所体现的带型特征。方法收集2018-2020年凉山州9733份经过HIV抗体确证试验阳性且为当年新报告感染者并保存完好的血样,用限制性抗原亲和力酶免试验(LAg-Avidity EIA)进行新发感染检测,比较新发感染者和长期感染者四种确证试剂带型特征。结果9733份新报告阳性样本中,7.61%(741/9733)判为HIV-1新发感染。MP-WB确证检测新发感染者确证带型gp41、p66、p51、p31、p55、p39、p17检出率明显低于长期感染者,且差异有统计学意义(Ρ<0.05)。IMT-WB确证检测新发感染者确证带型gp120、gp41、p66、p51、p31、p17检出率低于长期感染者,差异均有统计学意义(Ρ<0.05)。MK-LIA确证检测新发感染者确证带型gp120、p51、p31、p17检出率低于长期感染者,差异均有统计学意义(Ρ<0.05)。WT-RIBA确证检测新发感染者确证带型gp120、p31、p17检出率低于长期感染者,差异均有统计学意义(Ρ<0.05)。结论新发感染者和长期感染者四种确证试剂带型特征存在差异。缺失p31、p17提示为新发感染者的概率高。两种WB确认试剂当gp41、p66、p51条带缺失以及MK-LIA、WTRIBA缺失gp120时也对HIV-1新发感染判断有提示作用。Objective To analyze the correlation between the detection results of new HIV-1 infection and the detection bands of four confirmatory reagents in Liangshan Prefecture,and to understand the band characteristics of newly infected and long-term infected people in the four confirmatory reagents.Methods Blood samples from newly reported infected persons who were positive for the HIV antibody confirmatory test in Liangshan Prefecture from 2018 to 2020 were collected.New infections were detected by lag avidity EIA,and the band characteristics of four confirmatory reagents between newly infected persons and long-term infected persons were compared.Results Among 9733 newly reported positive samples,7.61%(741/9733)were judged as new HIV-1 infection.The detection rates of confirmatory bands gp41,p66,p51,p31,p55,p39,and p17 in new infections were significantly lower than those in long-term infections(R<0.05).The detection rates of confirmatory bands gp120,gp41,p66,p51,p31,and p17 in patients with new infection were lower than those in patients with long-term infection(P<0.05).The detection rates of confirmatory bands gp120,p51,P31 and p17 in new infections were lower than those in long-term infections(P<0.05).The detection rates of confirmatory bands gp120,P31,and p17 in new infections were lower than those in long-term infections(P<0.05).Conclusion There were differences in the band characteristics of four confirmatory reagents between new infection and long-term infection.Deletion of P31 and p17 suggested a high probability of new infection.When gp41,p66,and p51 bands are missing and MK LIA and WT RIBA are missing with two WB confirmation reagents,gp120 can also assist to diagnose the new HIV-1 infection.

关 键 词：1型艾滋病病毒感染者 新发感染检测 艾滋病病毒抗体确证检测带型 

分 类 号：R373.9[医药卫生—病原生物学]