生长素和独脚金内酯参与磷素调控水稻分蘖的机制初探  

Preliminary Study on Mechanism of Auxin and Strigolactone Involved in Phosphate-Modulated Tillering of Rice

作　　者：赵晨云[1] 郭小丽陈 亚轲 龚思远 李俊周[1] 赵全志[1] 孙虎威 ZHAO Chenyun;GUO Xiaoli;CHEN Yake;GONG Siyuan;LI Junzhou;ZHAO Quanzhi;SUN Huwei(Collaborative Innovation Center of Henan Grain Crops,Key Laboratory of Rice Biology in Henan Province,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南农业大学河南粮食作物协同创新中心/河南省水稻生物学重点实验室,河南郑州450046

出　　处：《河南农业科学》2022年第1期21-26,共6页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31601821)。

摘　　要：研究不同磷浓度[低磷(10μmol/L)和正常供磷(300μmol/L)]下水稻分蘖数和分蘖芽长度的变化及施加外源生长素萘乙酸(NAA,10 nmol/L)、生长素极性运输抑制剂萘基邻氨甲酰苯甲酸(NPA,0.5μmol/L)对水稻野生型及独脚金内酯信号转导关键基因突变体d14分蘖发生的影响,旨在揭示水稻分蘖对低磷胁迫的响应机制以及此过程中独脚金内酯和生长素的关系。结果表明,与正常供磷处理相比,低磷处理水稻的分蘖数显著降低,分蘖芽长度显著变短。在正常供磷条件下,施加外源NAA能够抑制水稻分蘖的发生;在低磷条件下,施加NPA不能促进水稻分蘖的发生。突变体d14对磷素不敏感,在正常供磷和低磷处理下均具有较多的分蘖数,说明D14基因在磷素调控水稻分蘖发生的过程中起重要作用。在正常供磷和低磷处理下,施加外源NAA均显著降低d14突变体分蘖数。综上,生长素和独脚金内酯均参与了低磷抑制水稻分蘖发生的过程,而在这个过程中两者可能存在一定的独立性。The changes of tiller number and tiller bud length of rice under low phosphate(LP,10μmol/L)and normal phosphate(NP,300μmol/L)conditions were studied,and the effect of application of exogenous auxin(naphthylacetic acid,NAA,10 nmol/L)and auxin polar transport inhibitor(N-1-naphthylphalamic acid,NPA,0.5μmol/L)on tillering of wild type and mutant of D14(key strigolactone signal transduction gene)was explored,so as to reveal the mechanism of rice tillering in response to LP stress and the relationship between strigolactone and auxin in this process.The results showed that,compared with NP treatment,the tiller number and tiller bud length of LP treatment significantly reduced.The application of NAA inhibited tillering of rice under NP condition,and the application of NPA could not promote tillering of rice under LP condition.The mutant d14 was insensitive to phosphate,and had more tillers under LP and NP conditions,suggesting that D14 played an important role in phosphate-modulated tillering of rice.The application of NAA significantly reduced tiller number of mutant d14 under LP and NP conditions.In conclusion,both auxin and strigolactone are involved in LP-inhibited tillering of rice,but they may have a certain independence in this process.

关 键 词：水稻 磷素 分蘖 生长素 独脚金内酯 D14基因 

分 类 号：S511[农业科学—作物学]