5-氮胞苷提高‘水晶’葡萄耐铝性的相关机制研究  

Mechanism of 5-Azacytidine on Improving Aluminum Tolerance of'Crystal'Grape

作　　者：徐仕琴 陈姣 张永福 莫丽玲 XU Shiqin;CHEN Jiao;ZHANG Yongfu;MO Liling(School of Agriculture and Life Sciences,Kunming University,Kunming 650214,China)

机构地区：[1]昆明学院农学与生命科学学院,云南昆明650214

出　　处：《中外葡萄与葡萄酒》2022年第2期20-25,共6页Sino-Overseas Grapevine & Wine

基　　金：国家自然科学基金(32060645);云南省教育厅科学研究基金项目(2022Y706)云南省高校高原特色果树优异种质资源挖掘与新品种选育科技创新团队建设经费的资助云南省地方本科高校基础研究联合专项重点项目;云南省地方高校联合专项重点项目(202101BA070001-036)云南省教育厅科学研究基金项目(2022Y703)。

摘　　要：为探讨5-氮胞苷对铝胁迫下葡萄植株生长及耐铝相关生理机制的响应,以‘水晶’葡萄扦插苗为试材,在20 mmol·L^(-1)硫酸铝胁迫下喷施不同浓度的5-氮胞苷,35 d后测定植株的形态指标及生理指标。结果表明,铝胁迫下植株茎叶萎蔫,株高和根系生物量下降,MDA剧增,叶绿素含量下降,SOD和POD的活性均被减弱,铝大幅度积累,抑制氮、磷、钾的转运。铝胁迫下喷洒5-氮胞苷可使株高、根鲜质量、根长、根体积提高,MDA含量降低的同时使得叶绿素含量增加,SOD和POD活性显著增强,铝的积累缓解,增强了氮、磷、钾的转运。其中,铝胁迫下喷洒100μmol·L^(-1)的5-氮胞苷的处理效果最显著,可减缓铝胁迫对‘水晶’葡萄植株的毒害从而提高其耐铝性,促进植株和根系生长,增强营养物质的积累以及膜脂过氧化程度。In order to explore the response of 5-azacytidine to the physiological mechanism of grape plant growth and aluminum tolerance under aluminum stress,the cuttings of'Crystal'grapevine were sprayed with different concentrations of 5-azacytidine under 20 mmol·L^(-1)aluminum sulfate stress.The morphological and physiological indexes of the plants were measured after 35 days.The results showed that the stem and leaf wilted,plant height and root biomass decreased under aluminum stress;The content of MDA increased sharply,the content of chlorophyll decreased,the activities of SOD and POD were weakened,the content of aluminum accumulated greatly,and the transport of nitrogen,phosphorus and potassium were inhibited.Spraying 5-azacytidine could increase plant height,root fresh weight,root length and root volume;The decrease of MDA content increased the chlorophyll content,significantly enhanced the activities of SOD and POD,alleviated the accumulation of aluminum content and enhanced the accumulation of nitrogen,phosphorus and potassium content.Among them,spraying 100μmol·L^(-1)5-azacytidine had the most significant effect under aluminum stress,which could slow down the toxicity of aluminum stress to'Crystal'grape plants and improve their aluminum tolerance,promote the growth of grape plants and roots under aluminum stress,accumulate nutrients and enhance the degree of membrane lipid peroxidation.

关 键 词：5-氮胞苷 水晶葡萄苗 铝胁迫 营养物质 

分 类 号：S663.1[农业科学—果树学]