生物法合成4-羟基异亮氨酸的代谢工程研究进展  

Advances on metabolic engineering for biosynthesis of 4-hydroxyisoleucine

作　　者：李洋 张稳杰 韩雨辰 翟懿雪 张成林[1] LI Yang;ZHANG Wenjie;HAN Yuchen;ZHIA Yixue;ZHANG Chenglin(College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学生物工程学院,天津300457

出　　处：《食品与发酵工业》2022年第5期281-288,共8页Food and Fermentation Industries

基　　金：国家自然科学基金项目(32170049,31300069);天津市高等学校大学生创新训练计划项目(202010057030);中国博士后科学基金项目(2017M611170,2018T110662)。

摘　　要：4-羟基异亮氨酸是一种非蛋白质氨基酸,因具有血糖浓度依赖的诱导胰岛素分泌等生物学活性,而被视为治疗糖尿病的潜在药物。近年来,生物法合成4-羟基异亮氨酸取得了一定的进展。文章概述了4-羟基异亮氨酸的功能、合成方法及其生物合成途径。在此基础上,从构建4-异亮氨酸合成途径、强化L-异亮氨酸合成、重构中心代谢途径等层面着重阐述了生物法合成4-羟基异亮氨酸的代谢工程策略,旨在为以4-羟基异亮氨酸为代表的高附加值氨基酸衍生物合成菌株代谢工程研究提供参考。4-Hydroxyisoleucine, a non-protein amino acid, has been considered as a potential alternative for diabetes-curing drugs.In recent years, considerable progress has been made on biosynthesis of 4-hydroxyisoleucine.In this study, the function, synthesis methods as well as biosynthesis pathway of 4-hydroxyisoleucine are illustrated.Furthermore, the metabolic engineering strategies are summarized, including construction of 4-hydroxyisoleucine biosynthesis pathway, enhancement of L-isoleucine synthesis and reconstruction of central pathways.It aims to provide a reference for metabolic engineering strains for producing value-added amino acid derivatives.

关 键 词：4-羟基异亮氨酸 生物合成 代谢工程 谷氨酸棒杆菌 大肠杆菌 

分 类 号：TQ460.1[化学工程—制药化工]