正常顺产婴儿出生6月内肠道菌群的发育特征  

The development characteristics of intestinal flora of healthy infant within 6 months after vaginal delivery

作　　者：林永创 李依米 吴璐璐 罗慧娟[1] 肖小敏[1] LIN Yongchuang;LI Yimi;WU Lulu;LUO Huijuan;XIAO Xiaomin(Department of Gynaecology and Obstetrics,the First Affiliated Hospital of Jinan University,Guangzhou 510632,Guangdong,China;Department of Gynaecology and Obstetrics,the Second People's Hospital of Yichang,Yichang 443000,Guangdong,China)

机构地区：[1]暨南大学附属第一医院妇产科,广东广州510632 [2]宜昌市第二人民医院妇产科,湖北宜昌443000

出　　处：《暨南大学学报（自然科学与医学版）》2022年第1期43-52,共10页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金项目(81771664)。

摘　　要：目的:探讨正常妊娠顺产婴儿胎粪菌群特点及出生6个月内婴儿肠道菌群发育的生理特点。方法:收集符合纳入标准的婴儿出生后第1次胎粪,第3天、14天及6个月的粪便标本12例,基于16SrRNA测序技术分析肠道菌群的多样性、物种差异。结果:(1)正常新生儿出生后第1次胎粪含丰富菌群,出生后第3天肠道菌群的α多样性在4个时期中最低;新生儿期(出生后第3及14天)肠道菌群的β多样性相似,而与第1次胎粪、出生后第6个月粪便标本之间差异具有统计学意义;(2)6个月内婴儿肠道菌群以变形菌门和放线菌门的变化最为显著,第1次胎粪中变形菌门相对丰度高达48%,出生后第3天时降至18.3%,主要是变形菌门中罗尔斯通菌属、不动杆菌属和鞘氨醇单胞菌属显著减少,此后至6月龄,变形菌门丰度保持相对稳定;而放线菌门相对丰度在出生后第1次胎粪中仅为1.9%,随年龄迅速上升,6月龄时高达43.1%;主要是双歧杆菌属显著增加。结论:肠道菌群定植可能始于宫内时期,胎粪中变形菌门占比非常高;出生后3天内肠道菌群处于比较脆弱的状态;6月内婴儿肠道菌群以双歧杆菌迅速增长为其发育特征。Objective:To investigate characteristics of meconium flora and physiological characteristics of gut flora in healthy infant within 6 months.Methods:Twelve healthy vaginally delivered newborns fecal samples at the 1 st/3 rd/14 th day and 6 months after birth were collected,to analyse the composition of the gut flora using the 16 SrRNA gene sequencing technology.Results:(1)By comparing the difference of the intestinal bacterial communities,theα-diversity at the 3 rd day after birth was significantly lower than that of the first meconium after birth.Theβ-diversity of intestinal bacterial in the neonatal period(3 rd day and 14 th day after birth)was similar,and the difference was statistically significant between the 1 st meconium and the 6 th month after birth.(2)Proteobacteria and Actinobacteria showed the most significant changes in the intestinal flora of infants within 6 months after birth.The relative abundance of the Proteobacteria was 48%at birth,then it rapidly decreased to 18.3%at the 3 rd day,the decreased relative abundance of Acinetobacteria,Sphingomonas and Ralstonia were responsible for the change of Proteobacteria.After that,it remained relatively stable.As the neonates grew up,the relative abundance of Actinobacteria increased.At the 1 st day after birth,it was only 1.9%in the fecal bacterial communities.6 months later,it was up to 43.1%,because of the relative abundance of Bifidobacterium increased rapidly.The relative abundance of Bifidobacterium increased rapidly.Conclusion:The intestinal flora of neonates probably colonized at intrauterine period,which was in a fragile state and changed rapidly at the first 3 days.The colonization of intestinal flora may begin in the intrauterine period,and the proportion of Proteobacteria in meconium was very high.The intestinal flora was in a relatively fragile state within 3 days after birth.Rapid growth of bifidobacteria was the development characteristics of infant gut flora within 6 months.

关 键 词：婴儿肠道菌群 发育特征 物种差异 

分 类 号：R71[医药卫生—妇产科学]