沉默GbGSTU7基因对海岛棉枯萎病抗性的影响  

Effect of Silencing GbGSTU7 Gene on the Resistance to Fusarium Wilt of Gossypium barbadense

作　　者：邓晓娟 陆小双 陈琴[1] 张梦洁 曲延英[1] DENG Xiao-Juan;LU Xiao-Shuang;CHEN Qin;ZHANG Meng-Jie;QU Yan-Ying(Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆农业大学,乌鲁木齐830052

出　　处：《农业生物技术学报》2022年第3期434-441,共8页Journal of Agricultural Biotechnology

基　　金：转基因生物新品种培育重大专项课题研究计划(2016ZX08005-005);国家自然科学基金(31860394)。

摘　　要：GSTU7基因编码谷胱甘肽转移酶(glutathiones transferases,GSTs),本课题组前期通过对枯萎病菌(Fusarium oxysporum f.sp.vasinfectum)感染的海岛棉(Gossypium barbadense)转录组数据分析发现,GbGSTU7(GenBank No.GB_A03G0575)基因响应抗枯萎病过程。为了验证GbGSTU7基因在海岛棉抗枯萎病中的作用,本研究利用烟草脆裂病毒(Tobacco tattle virus,TRV)诱导的基因沉默系统(virus-induced gene silencing,VIGS)沉默海岛棉抗病材料'06-146'中的GbGSTU7基因,通过qPCR技术分析GbGSTU7基因的表达情况;采用枯萎病7号生理小种侵染GbGSTU7基因沉默植株,以研究基因沉默植株的抗病性,同时对海岛棉谷胱甘肽过氧化物酶的活力进行测定、分析。结果显示,诱导植株GbGSTU7基因的沉默效率为74%,对棉花枯萎病的病情指数升高了31.4,可溶性蛋白含量上升了3倍,谷胱甘肽过氧化物酶活性降低了2倍。表明GbGSTU7基因在海岛棉的抗枯萎病反应中具有重要作用,GbGSTU7的沉默影响了蛋白转运,与谷胱甘肽过氧化物酶协同起到抵御病菌的侵染。本研究为深入探讨GbGSTU7基因参与海岛棉枯萎病中的抗性机制提供了参考依据。The GSTU7 gene encodes glutathione transferase.In the previous research,this group found out that GbGSTU7(GenBank No.GB_A03G0575)gene responds to the process of resistance to Fusarium wilt.In order to verify the GbGSTU7 gene in the resistance of Gossypium barbadense to Fusarium wilt,the Tobacco rattle virus-mediated virus induced gene silencing system was used to silence the GbGSTU7 gene in the disease-resistant material of Gossypium barbadense'06-146',and qPCR technology was used to analyze the expression of GbGSTU7 gene.The physiological race 7 of Fusarium wilt was used to infect the the GbGSTU7 gene silenced plants to study their disease resistance and measure the glutathione peroxidase activity of Gossypium barbadense.The results showed that the silencing efficiency of GbGSTU7 gene was 74%,and the disease index for cotton Fusarium wilt increased 31.4,the soluble protein content increased by 3 times,and the activity of glutathione peroxidase decreased by 2 times,indicating that the GbGSTU7 gene played an important role in the anti-fusarium wilt reaction of Gossypium barbadense.The silencing of GbGSTU7 affected protein transport and cooperated with glutathione peroxidase to resist infection by pathogens.This study provides a reference for the in-depth study of the mechanism of the GbGSTU7 gene involved in the resistance to Fusarium wilt of Gossypium barbadense.

关 键 词：海岛棉 谷胱甘肽转移酶 病毒诱导的基因沉默系统 枯萎病 

分 类 号：S56[农业科学—作物学]