绵羊MYF5基因多态性及其与生长性状的关联分析  

Polymorphism of MYF5 Gene and Its Association Analysis with Growth Traits in Sheep(Ovis aries)

作　　者：孟科 张天闻 梁鹏 邵顺成 邹诗凡 荣轩 强浩 冯登侦[1] MENG Ke;ZHANG Tian-Wen;LIANG Peng;SHAO Shun-Cheng;ZOU Shi-Fan;RONG Xuan;QIANG Hao;FENG Deng-Zhen(School of Agriculture,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学农学院,银川750021

出　　处：《农业生物技术学报》2022年第3期496-505,共10页Journal of Agricultural Biotechnology

基　　金：宁夏回族自治区农业育种专项(2019NYYZ0403);宁夏回族自治区自然科学基金(2020AAC03082)。

摘　　要：肌源性因子5(myogenic factor 5,MYF5)被认为是影响肌肉生长发育的重要基因。本研究旨在探究MYF5基因的遗传变异对绵羊(Ovis aries)生长性状的影响,以期为优质肉羊新品种(系)培育提供有效的分子遗传标记。利用液相捕获测序技术获得MYF5基因在杜泊羊、小尾寒羊和滩羊共91只羊中的变异位点,筛选出具有多态性且品种间差异显著的3个SNP位点(124510044,124509770和124507708)。针对筛选出的位点利用飞行质谱对3个绵羊品种杂交后代的383只绵羊进行检测,并与初生和3月龄的生长性状进行关联分析。结果显示:124510044位点检测出3种基因型,在所有群体中均处于中度多态(0.25≤PIC<0.50);124509770位点检测出3种基因型,在H1代群体中处于低度多态(PIC<0.25),在其他群体中处于中度多态(0.25≤PIC<0.50);124507708位点只检测出缺失1种基因型。在H_(2)代群体中,124510044位点CT基因型个体的3月龄体高显著高于TT基因型(P<0.05),124509770位点CG基因型个体3月龄体高显著高于GG基因型(P<0.05);在不考虑世代的情况下,GG基因型个体3月龄胸围显著高于CC基因型(P<0.05)。124510044和124509770位点为完全连锁不平衡,产生4种单倍型和5种双倍型;双倍型H4H4个体初生和3月龄的胸围显著高于H1H1(P<0.05)。本研究结果表明,MYF5基因的遗传变异对绵羊的生长性状有影响,124510044和124509770位点可以作为肉羊生长发育和产肉性能的潜在候选遗传标记,本研究为MYF5基因在肉羊的选种选育提供了重要参考,为肉羊的分子标记辅助育种中提供了理论依据。The myogenic factor 5(MYF5)gene is considered to be an important gene affecting muscle growth and development.The aim of this study was to investigate the effect of genetic variation of MYF5 gene on growth traits in sheep(Ovis aries),in order to provide effective molecular genetic markers for the breeding of new breeds(lines)of high quality meat sheep.Three SNP loci(124510044,124509770 and 124507708)with polymorphism and significant interbreed differences were screened.The selected loci were examined by flight mass spectrometry in 383 sheep from the 3 crosses and correlated with growth traits at birth and 3 months of age.The results showed that locus 12451044 detected 3 genotypes,which were moderately polymorphic(0.25≤PIC<0.50)in all populations;locus 124509770 detected 3 genotypes,which were low polymorphic(PIC<0.25)in the H1 generation population and moderately polymorphic(0.25≤PIC<0.50)in the other populations.Only one genotype deletion was detected at locus 124507708.In the H_(2) generation population,individuals with CT genotype at locus 124510044 had significantly higher body height at 3 months of age than TT genotype(P<0.05),and individuals with CG genotype at locus 124509770 had significantly higher body height at 3 months of age than GG genotype(P<0.05);individuals with GG genotype had significantly higher chest circumference at 3 months of age than CC genotype,regardless of generation(P<0.05).124510044 and 124509770 loci were complete linkage disequilibrium,producing 4 haplotypes and 5 diploids;individuals with diploid H4H4 had significantly higher chest circumference at birth and at 3 months of age than H1H1(P<0.05).The results of this study showed that genetic variation in the MYF5 gene has an effect on growth traits in sheep,and the loci 124510044 and 124509770 can be used as potential candidate genetic markers for growth and development and meat production performance in meat sheep.This study provides an important reference for the MYF5 gene in the selection and breeding of meat sheep,and provid

关 键 词：绵羊 肌源性因子5 (MYF5)基因 多态性 生长性状 关联分析 

分 类 号：S826[农业科学—畜牧学]