早期补饲开食料对羔羊瘤胃组织转录组的影响  

Effects of Early Supplementation with Starter on Rumen Tissue Transcriptome in Lambs(Ovis aries)

作　　者：汪晓娟[1] 吕凤 庞鑫 刘国华[1] 赵海碧 WANG Xiao-Juan;LYU Feng;PANG Xin;LIU Guo-Hua;ZHAO Hai-Bi(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学动物科学技术学院,兰州730070

出　　处：《农业生物技术学报》2022年第3期517-527,共11页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金地区项目(31760682)。

摘　　要：瘤胃是反刍动物重要的消化器官,早期补饲开食料可以满足羔羊(Ovis aries)快速生长的营养需要,并促进瘤胃早期发育。本研究通过研究羔羊补饲开食料对瘤胃组织基因表达的影响,旨在阐明开食料对瘤胃发育的作用机理。选取健康的7日龄湖羊公羔22只,随机分为对照组与补饲组,对照组饲喂代乳粉,补饲组在饲喂代乳粉的基础上补饲开食料。28日龄屠宰,采集瘤胃腹囊组织,进行转录组测序分析。结果显示:对照组与补饲组分别获得61.70和58.95 Gb的有效数据,与参考基因组的比对效率在87.85%~89.45%;与对照组相比,补饲后筛选出868个差异表达基因,其中上调基因464个,下调基因404个,主要富集于酶活性、离子通道、膜运输等GO功能及甾类激素生物合成、花生四烯酸代谢、过氧化物酶体增殖物激活受体信号通路、脂肪细胞脂解作用的调节、环腺苷酸等KEGG信号通路。关联性分析显示,含IQ基元GTP酶激活蛋白2重组蛋白(recombinant IQ motif containing GTPase activating protein 2,IQGAP2),溶质载体家族16成员1重组蛋白(recombinant solute carrier family 16,member 1,SLC16A1),锚蛋白重复结构域45(ankyrin repeat domain-containing protein 45,ANKRD45),3-羟基-3-甲基戊二酰辅酶A合成酶(3-hydroxy-3-methylglutaryl-CoA synthase 2,HMGCS2),磷酸烯醇式丙酮酸羧化酶2(phosophoenolpyruvate carboxykinase 2,PCK2)均与瘤胃乳头长度、乙酸、丙酸、丁酸和总挥发性脂肪酸(total volatile fatty acid,TVFA)显著正相关(P<0.05);而神经酰胺合酶3(ceramide synthase 3,CERS3),角蛋白4重组蛋白(recombinant keratin 4,KRT4),可溶性载质转运蛋白22A17抗体(solute carrier family 22A17,SLC22A1),胰岛素样生长因子结合蛋白6(recombinant insulin like growth factor binding protein 6,IGFBP6)均与其呈显著负相关(P<0.05)。说明羔羊补饲开食料后可能是通过调控与瘤胃上皮增殖和养分代谢相关基因的表达量而促进了瘤胃发育�Rumen is an important digestive organ of ruminants,early supplementation with starter can meet the nutritional needs of rapid growth of lambs(Ovis aries)and promote the early development of rumen.This study was conducted to investigate the effects of supplementation with starter on gene expression in rumen tissues of lambs to clarify the possible mechanism of starter on rumen development.Twenty-two healthy and disease-free male Hu lambs(birth weight=(3.65±0.49)kg)were randomly divided into 2 groups,control group and supplementary feeding group.Control group was fed with milk replacer,while supplementary feeding group was fed with milk replacer and starter.At the age of 28 days,rumen pouch tissues were collected for transcriptome sequencing analysis.The result showed that 61.70 and 58.95 Gb of clean reads were obtained in control group and supplementary feeding group,respectively,and the comparison efficiency with the reference genome was 87.85%~89.45%.Compared with the control group,868 differentially expressed genes were screened after supplementary feeding,among which 464 genes were up-regulated and 404 genes were down-regulated.And it was mainly concentrated in GO functions such as enzyme activity,ion channel and membrane transport and KEGG signaling pathways such as steroid hormone biosynthesis,arachidonic acid metabolism,peroxisome proliferator-activated receptor(PPAR)signaling pathway,regulation of lipolysis in adipocytes and cyclic adenosine monophosphate(cAMP)signaling pathway.Correlation analysis showed that recombinant IQ motif containing GTPase activating protein 2(IQGAP2),recombinant solute carrier family 16,member 1(SLC16A1),ankyrin repeat domain-containing protein 45(ANKRD45),3-hydroxy-3-methylglutaryl-CoA,synthase 2(HMGCS2),phosophoenolpyruvate carboxykinase 2(PCK2)were significantly positively correlated with the length of rumen papilla,the concentration of acetate,propionate,butyrate and total volatile fatty acid(TVFA)(P<0.05),while ceramide synthase 3(CERS3),recombinant keratin 4(KRT4),solute c

关 键 词：开食料 羔羊 瘤胃 基因表达 转录组 

分 类 号：S826[农业科学—畜牧学]