MALAT1通过靶向miR-204调控TFAM表达对皮肤鳞状细胞癌增殖及凋亡的影响  

Effect of MALAT1 on the proliferation and apoptosis of skin squamous cell carcinoma by regulating TFAM expression by targeting miR-204

作　　者：范荣辉[1] 徐子寒[1] 慕生枝[1] 李罡 Fan Rong-hui;Xu Zi-han;Mu Sheng-zhi;Li Gang(Burn and Plastic Surgery,Shaanxi Provincial People's Hospital,Xi'an 710032,China;School of Basic Medicine,Xi'an Jiaotong University,Xi'an 710061,China)

机构地区：[1]陕西省人民医院烧伤整形医学美容外科,陕西西安710032 [2]西安交通大学基础医学院,陕西西安710061

出　　处：《兰州大学学报（医学版）》2022年第2期9-15,共7页Journal of Lanzhou University（Medical Sciences）

摘　　要：目的 探讨MALAT1靶向miR-204/线粒体转录因子A (TFAM)轴对皮肤鳞状细胞癌恶性生物学行为的影响。方方法 lncRNA芯片筛选皮肤鳞状细胞癌中差异表达的lncRNA;qRT-PCR检测用于分析MALAT1与miR-204的表达;免疫组织化学法与Western blotting检测TFAM的表达;si-阴性对照、si-MALAT1、miR-204类似物、类似物阴性对照、miR-204抑制剂、抑制剂阴性对照、pcDNA3.1质粒与pcDNA3.1-TFAM质粒分别转染皮肤鳞状细胞癌SCL-1细胞;荧光素酶活性分析实验用于检测MALAT1与miR-21,TFAM与miR-21的靶向关系;细胞克隆形成实验及流式细胞术用于检测SCL-1细胞的增殖及凋亡。结果 MALAT1与TFAM在皮肤鳞状细胞癌组织及细胞中高表达,而miR-204在皮肤鳞状细胞癌组织及细胞中低表达。沉默MALAT1或过表达miR-204,均可抑制TFAM蛋白表达,抑制SCL-1细胞增殖,促进细胞凋亡。结论 MALAT1靶向miR-204/TFAM轴影响皮肤癌细胞增殖及凋亡。Objective To investigate the effect of MALAT1 targeting miR-204/TFAM axis on proliferation and apoptosis of skin squamous cell carcinoma. Method lncRNA array was used to detect the differential expression of lncRNA in skin squamous cell carcinoma;qRT-PCR detection was performed to analyze the expression of MALAT1 and miR-204;Immunohistochemistry and Western blotting were used to detect the expression of TFAM;si-NC, si-MALAT1, miR-204 mimic, mimic NC, miR-204 inhibitor, inhibitor NC, pcDNA3.1 plasmid and pcDNA3.1-TFAM plasmid were respectively transfected into skin squamous cell carcinoma SCL-1 cell;Luciferase activity analysis experiment was used to detect the targeting relationship between MALAT1 and miR-21, TFAM and miR-21;Cell clone formation experiment and flow cytometry were evaluated to detect the proliferation and apoptosis of SCL-1 cells. Results MALAT1 and TFAM were highly expressed in skin squamous cell carcinoma tissue and cells, while miR-204 was low expressed in skin squamous cell carcinoma tissue and cells;silencing MALAT1 or overexpression of miR-204, inhibited TFAM protein expression, inhibited SCL-1 cell proliferation, and promoted apoptosis. Conclusion MALAT1 regulated the cell proliferation and apoptosis by targeting the miR-204/TFAM axis in skin squamous cells.

关 键 词：MALAT1 miR-204 线粒体转录因子A 皮肤鳞状细胞癌 细胞增殖 细胞凋亡 

分 类 号：R446.1[医药卫生—诊断学]