TNFAIP3基因多态性与老年性骨质疏松性骨折及骨代谢指标的相关性研究  

Correlation of TNFAIP3 gene polymorphism with osteoporotic fractures and bone metabolism in elderly patients

作　　者：杜保卫[1] 韩鹏飞 刘宇 李宏倬[1] Du Baowei;Han Pengfei;Liu Yu;Li Hongzhuo(Department of Orthopedics,Heping Hospital,Changzhi Medical College,Changzhi 046000,China)

机构地区：[1]山西省长治医学院附属和平医院骨科,长治046000

出　　处：《中华内分泌外科杂志》2022年第1期103-107,共5页Chinese Journal of Endocrine Surgery

基　　金：山西省卫生健康委科研课题 (2020133)。

摘　　要：目的探讨TNFAIP3基因多态性与老年性骨质疏松性骨折及骨代谢指标的相关性。方法收集2019年1月至2021年6月山西省长治医学院附属和平医院就诊的115例老年性骨质疏松性骨折患者作为观察组,同时选择120例与观察组性别、年龄和体质量指数相匹配的老年骨质疏松无骨折患者作为对照组。记录所有研究对象的血钙、血磷、碱性磷酸酶、25-羟基维生素D等骨代谢指标的水平。采用毛细管电泳和片段分析(SNaPshot)技术进行分析TNFAIP3基因上rs10499194和rs13207033位点的基因型。应用实时荧光定量聚合酶链式反应测定外周血中的TNFAIP3基因mRNA相对表达量。结果观察组和对照组的rs10499194、rs13207033基因型分布和基因频率比较差异均有统计学意义(P均<0.05)。对于rs10499194位点,相较于野生型CC基因型携带者,CT基因型携带者发生骨折的风险显著增大[OR=3.25(1.22~8.65),P=0.014];显性模型差异也有统计学意义(P=0.003)。对于rs13207033位点,相较于野生型GG基因携带者,GA基因携带者发生骨折的风险显著增大[OR=3.78(1.19~11.95),P=0.016];显性模型差异有统计学意义(P=0.009)。rs13207033位点AA+GA组的血钙水平显著高于GG组(P=0.006)。观察组TNFAIP3基因mRNA表达水平为(1.41±0.09),显著低于观察组的表达水平(2.07±0.12)(t=6.69,P<0.001)。rs10499194位点TT+CT组患者的TNFAIP3基因mRNA表达水平为(1.35±0.11),显著低于CC组的表达水平(1.43±0.13)(t=2.82,P=0.007)。结论TNFAIP3基因多态性与老年性骨质疏松性骨折发生及骨代谢指标有关,并可影响基因表达水平。Objective To investigate the correlation between TNFAIP3 gene polymorphism and osteoporotic fractures in the elderly and bone metabolism indexes.Methods A total of 115 patients with senile osteoporotic fractures admitted to Peace Hospital Affiliated to Shanxi Changzhi Medical College from Jan.2019 to Jun.2021 were enrolled as the observation group,and 120 patients with senile osteoporotic fractures matched with gender,age and body mass index of the observation group were selected as the control group.The levels of blood calcium,blood phosphorus,alkaline phosphatase,25-hydroxyvitamin D and other bone metabolism indexes of all subjects were recorded.The genotypes of rs10499194 and rs13207033 in TNFAIP3 gene were analyzed by capillary electrophoresis and fragment analysis(SNaPshot).The mRNA relative expression level of TNFAIP3 gene in peripheral blood of all subjects was determined by quantitative real-time fluorescence quantitative polymerase chain reaction.Results There were statistically significant differences in genotype distribution and gene frequency of RS10499194 and RS13207033 between the observation group and the control group(P<0.05).For rs10499194,CT genotype carriers had a significantly higher risk of fracture than wild-type CC genotype carriers[OR=3.253(1.223-8.652),P=0.014].The dominant model was also statistically significant(P=0.003).For rs13207033,GA carriers had a significantly higher risk of fracture than wild-type GG carriers[OR=3.775(1.192-11.952),P=0.016].The dominant model was statistically significant(P=0.009).The blood calcium level in AA+GA group was significantly higher than that in GG group at rs13207033 site(P=0.006).The mRNA expression level of TNFAIP3 gene in the observation group was 1.41±0.09,which was significantly lower than that in the observation group(2.07±0.12,t=6.69,P<0.001).TNFAIP3 mRNA expression level of rs10499194 site TT+CT group was 1.35±0.11,significantly lower than CC group 1.43±0.13(t=2.82,P=0.007).Conclusion The polymorphism of TN-FAIP3 gene is related to the oc

关 键 词：老年性骨质疏松性骨折 TNFAIP3 单核苷酸多态性 骨代谢 

分 类 号：R658.3[医药卫生—外科学] R738.1[医药卫生—临床医学]