甘蔗AP2/ERF基因家族的鉴定及表达  

Identification and expression analysis of AP2/ERF gene family in sugarcane

作　　者：陈玉凤[1] 李竹[1] 苏炜华[1] 孙婷婷[1] 汪洲涛 岑光莉 阙友雄[1,2] 蒙寅恬 许莉萍[1,2] 苏亚春[1,2] CHEN Yufeng;LI Zhu;SU Weihua;SUN Tingting;WANG Zhoutao;CEN Guangli;QUEYouxiong;MENG Yintian;XU Liping;SU Yachun(Key Laboratory of Sugarcane Biology and Genetic Breeding(Fujian)of Ministry of Agriculture and Rural Affairs,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Key Laboratory of Crop Genetics and Breeding and Comprehensive Utilization,Ministry of Education,College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学农业农村部福建甘蔗生物学与遗传育种重点实验室,福州350002 [2]福建农林大学教育部作物遗传育种与综合利用重点实验室,福州350002

出　　处：《应用与环境生物学报》2022年第1期67-81,共15页Chinese Journal of Applied and Environmental Biology

基　　金：国家重点研发计划项目(2018YFD1000503,2019YFD1000500);国家自然科学基金项目(31671752);国家糖料产业技术体系(CARS-17)资助。

摘　　要：AP2/乙烯应答元件结合因子(APETALA2/ethylene-responsive element binding factor,AP2/ERF)是植物中最大的转录因子家族之一,在植物的生长发育、生物和非生物逆境响应中发挥着重要作用.为了解甘蔗AP2/ERF基因家族的生物学特性,利用生物信息学方法从甘蔗野生种割手密(Saccharum spontaneum)基因组挖掘获得121条SsAP2/ERF基因序列,对其编码蛋白的理化性质、系统进化树、染色体定位和基因结构等进行分析.此外,从我国大陆主栽甘蔗品种ROC22中克隆获得1条SsAP2/ERF-107的同源基因ScDREB2B-2,并分析其表达模式.结果显示,SsAP2/ERF家族基因分布于31条割手密染色体上,且存在基因聚集现象,属于AP2、ERF和RAV亚家族的基因数目分别为28、86和7.SsAP2/ERF-001-SsAP2/ERF-121蛋白之间的分子量差异较大,介于16548.18-609263.26,不含信号肽,且99.17%无跨膜螺旋结构,含有3-10个保守基序和1个以上的外显子,推测大多数蛋白为定位于细胞核或细胞质的不稳定的亲水性蛋白.SsERF亚家族包含42个SsDREBs和44个SsERFs亚组成员,其AP2结构域的第14位氨基酸残基具有极高的保守性.克隆获得DREB(A-2)亚组成员SsAP2/ERF-107的同源基因ScDREB2B-2,两者氨基酸序列相似性达97.58%.qRT-PCR分析显示,ScDREB2B-2基因在ROC22中受聚乙二醇(PEG)、NaCl和脱落酸(ABA)诱导上调表达;在品种Badila中仅受PEG和NaCl诱导上调表达,而在ABA处理下的基因表达量不变,表明该基因在ROC22中可能通过ABA依赖性途径调节应答干旱和盐胁迫,而在Badila中可能通过非ABA依赖性途径调节响应干旱和盐胁迫.本研究结果可为探究甘蔗SsAP2/ERF基因家族的结构和功能提供科学依据.AP2/ERF(APETALA2/ethylene responsive element binding factor)is one of the largest transcription factor families in plants and plays an important role in plant growth,development,and biotic and abiotic stress responses.To understand the biological characteristics of the AP2/ERF gene family in sugarcane,121 SsAP2/ERF genes were obtained from the genome of the sugarcane wild species Saccharum spontaneum using a bioinformatics method.The physicochemical properties,phylogenetic tree,chromosomal localization,and gene structure of these SsAP2/ERFs were analyzed.A homologous gene of SsAP2/ERF-107,ScDREB2 B-2,was cloned from the prevalent sugarcane cultivar ROC22 in the mainland of China,and its expression pattern was detected.The results showed that the SsAP2/ERF family genes were distributed on 31 chromosomes of S.spontaneum with the phenomenon of gene aggregation.The number of genes belonging to the AP2,ERF,and RAV subfamilies were 28,86,and seven,respectively.The molecular weight of SsAP2/ERF-001–SsAP2/ERF-121 proteins varied substantially,ranging from 16548.18 to 609263.26.In SsAP2/ERF family proteins,there were no signal peptides,and 99.17%of the proteins had no transmembrane helix structure.Furthermore,SsAP2/ERF family proteins contained 3-10 conserved motifs as well as more than one exon.It was speculated that most of the SsAP2/ERF proteins were unstable,and hydrophilic proteins were located in the nucleus or cytoplasm.The SsERF subfamily contained 42 SsDREBs and 44 SsERFs subgroup members,and the 14 th amino acid residue of the AP2 domain was highly conserved.ScDREB2 B-2,a member of the DREB(A-2)subgroup,was cloned.The amino acid sequence similarity of ScDREB2 B-2 and its homologous gene,SsAP2/ERF-107,was 97.58%.qRT-PCR analysis demonstrated that the expression of ScDREB2 B-2 gene could be upregulated by polyethylene glycol(PEG),NaCl,and abscisic acid(ABA)in ROC22 but only by PEG and NaCl in variety Badila,suggesting that this gene may regulate the response to drought and salt stress through an ABA-dependent pa

关 键 词：割手密 AP2/ERF基因家族 ERF亚家族 全基因组分析 表达模式 

分 类 号：S566.1[农业科学—作物学]