缓丘地带黄河镇水库微生物群落多样性特征  

Characteristics of microbial community diversity in the gentle hilly area of Huanghe Town Reservoir

作　　者：文新宇 谌书[1] 杨远坤 王彬[1] 李函珂 刘杰 唐阳 封小倩 WEN Xinyu;CHEN Shu;YANG Yuankun;WANG Bin;LI Hanke;LIU Jie;TANG Yang;FENG Xiaoqian(School of Environment and Resource,Southwest University of Science and Technology,Mianyang 621010,China)

机构地区：[1]西南科技大学环境与资源学院,绵阳621010

出　　处：《应用与环境生物学报》2022年第1期167-174,共8页Chinese Journal of Applied and Environmental Biology

基　　金：四川省重大专项计划(2018SZDZX0026);四川省重点研发项目(2020YFS0306,2019YFS0055)资助。

摘　　要：微生物群落是缓丘地带湖库水生生态系统的重要组成部分,为了解析水体营养与缓丘地带湖库微生态的耦合变化关系,以四川省内江市典型缓丘地带富营养湖库黄河镇水库为研究对象,采集4个不同富营养化区域的水样,通过高通量测序技术分析不同采样点营养水平对微生物群落结构特征和多样性的影响.结果表明,当水体综合营养指数(TLI)为40-60,营养程度较低,水质较好时,轮虫门、绿弯菌门、α变形菌等微生物相对丰度较高;当水体综合营养指数大于70,营养程度较高时,水体中的微生物群落多样性和丰富度增加,放线菌门、绿藻门、硅藻门等占据主导;当营养程度进一步增加,蓝藻暴发生长时,水体中的微生物群落多样性和丰富度会大幅度降低,同时,拟杆菌门、厚壁菌门、黄杆菌属等微生物开始大量增殖.上述结果表明水体营养程度对水库微生态有重要影响,微生物群落丰度和多样性差异是判断水体富营养程度的重要生态学指标.The microbial community is an important part of the aquatic ecosystem of lakes and reservoirs in gentle hilly zones.It is closely related to the nutrient profile of the water body.The analysis of the relationship between nutrient profile and the micro-ecological coupling changes of lakes and reservoirs is of great significance for controlling the eutrophication of lakes and reservoirs.We studied the eutrophic lake reservoir in the gentle hilly area of Huanghe Town Reservoir.Using high-throughput sequencing,the water quality and microbial community structure changes at the four sampling points were analyzed.Additionally,the changes in the correlation characteristics between nutrient profile of the water and microbial diversity were explored.The results showed that when the comprehensive trophic level index(TLI)was between 40 and 60,the structure of eukaryotic plankton and bacterial communities began to recover,and the relative abundance of microorganisms(such as Rotifers,Chloroflexi,andα-proteobacteria)began to increase.The diversity and abundance of eukaryotic plankton and bacterial communities increased in the reservoir when the comprehensive(TLI)was greater than 70.In addition,microorganisms such as Actinobacteria,Chlorophyta,and Diatomea began to proliferate in large quantities.As the nutrient levels increased further and cyanobacteria bloomed,the diversity and abundance of eukaryotic plankton and bacterial communities decreased markedly and Bacteroides,Firmicutes,and Flavobacterium began to proliferate considerably.These results indicate that the water nutrient profile has an important impact on the micro-ecology of the reservoir,and the abundance and diversity of microbial communities are important ecological indicators for assessing the degree of eutrophication of water bodies.

关 键 词：缓丘地带湖库 富营养化 高通量测序 微生物群落结构 微生物群落多样性 蓝藻暴发 

分 类 号：Q938[生物学—微生物学]