IL-17诱导的p300调控NSCLC细胞迁移、侵袭和MMP2表达的作用  

IL-17-induced p300 regulates the migration,invasion and MMP2 production of NSCLC cells

作　　者：李雅 葛文 张志伟 蒋馨怡 何庆玲 阮玉婷 吴宁霞 应帅 王伟民 张婧[1] 邱文[1] 王迎伟[1] 赵晨卉[2] LI Ya;GE Wen;ZHANG Zhiwei;JIANG Xinyi;HE Qingling;RUAN Yuting;WU Ningxia;YING Shuai;WANG Weimin;ZHANG Jing;QIU Wen;WANG Yingwei;ZHAO Chenhui(Department of Immunology,Nanjing Medical University,Nanjing 211166;Department of Oncology,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]南京医科大学免疫学系,江苏南京211166 [2]南京医科大学第一附属医院肿瘤科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2022年第2期160-165,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81902878)。

摘　　要：目的:探讨IL-17刺激非小细胞肺癌(non-small cell lung cancer,NSCLC)细胞上调腺病毒E1A相关300 kDa蛋白(adenoviral E1A binding protein of 300 kDa,p300)调控其细胞迁移、侵袭及生成基质金属蛋白酶2(matrix metalloproteinase 2,MMP2)的作用。方法:用IL-17刺激NSCLC细胞(H1299)后行划痕和Transwell实验观察细胞迁移和侵袭的变化。然后用Western blot检查p300和MMP2蛋白的表达。同时,将p300过表达质粒(pcDNA3.1/p300)或shp300干扰质粒分别转染H1299细胞(后者再行IL-17刺激),用前述方法测定细胞迁移、侵袭和MMP2的表达。结果:用IL-17刺激H1299细胞后能显著诱导其迁移和侵袭,并上调p300和MMP2的表达。过表达p300可增强细胞的迁移与侵袭,并提高MMP2的生成,但沉默p300基因后由IL-17诱导的细胞迁移和侵袭能力及MMP2的表达均显著降低。结论:IL-17上调的p300能促进H1299细胞的迁移与侵袭以及MMP2基因的表达。Objective:This study aims to investigate the effects of adenovirus E1A associated 300 kDa protein(p300)on the migration,invasion and matrix metalloproteinase-2(MMP2)expression in non-small cell lung cancer(NSCLC)cells stimulated by IL-17.Methods:After NSCLC cells(H1299)incubated with IL-17,scratch and transwell experiments were performed to observe the cell migration and invasion.Then the p300 and MMP2 protein expression was detected using Western blot.Meantime,p300 overexpression plasmids(pcDNA3.1/p300)or short hairpin RNA(shp300)plasmids were transfected into H1299 cells(the latter was stimulated with IL-17),respectively,and the cell migration,invasion and MMP2 synthesis were again examined by the same method described above.Results:The migration and invasion as well as the expression of p300 and MMP2 were obviously elevated in H1299 cells treated by IL-17.And p300 overexpression significantly promoted the cell migration,invasion,and MMP2 expression.While p300 gene knockdown by shp300 led to the decrease of migration,invasion and MMP2 production in H1299 cells induced by IL-17.Conclusion:IL-17-upregulates p300 in H1299 cells which enhances the cell migration,invasion and MMP2 expression.

关 键 词：IL-17 NSCLC P300 MMP2 细胞迁移和侵袭 

分 类 号：R392.12[医药卫生—免疫学]