多组学技术在红豆杉研究中的应用  

Application of Multi-omics Technique in Taxus spp. Research

作　　者：丰美静 张恺恺 陈段芬[2] 贺磊[1] 邱德有[1] 杨艳芳[1] Feng Meijing;Zhang Kaikai;Chen Duanfen;He Lei;Qiu Deyou;Yang Yanfang(Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration,State Key Laboratory of Tree Genetics and Breeding,Research Institute of Forestry,Chinese Academy of Forestry,Beijing,100091;College of Horticulture,Hebei Agricultural University,Baoding,071001)

机构地区：[1]中国林业科学研究院林业研究所,林木遗传育种国家重点实验室,国家林业局林木培育重点实验室,北京100091 [2]河北农业大学园艺学院,保定071001

出　　处：《分子植物育种》2022年第3期840-846,共7页Molecular Plant Breeding

基　　金：本研究由国家林业和草原局科技发展中心项目(KJZXSA202037);国家自然科学基金项目(31570675;31670676)共同资助。

摘　　要：组学技术及多组学联合分析已成为动植物分子生物学研究常用的技术手段,以转录组学、蛋白质组学及代谢组学为基础的组学技术,已被广泛应用于红豆杉的各项研究中。综述了近年来国内外有关基因组、转录组、蛋白质组与代谢组等组学技术在红豆杉分子标记开发、紫杉醇生物合成、功能基因、抗寒能力以及紫杉醇生物合成相关代谢物中的应用,讨论了这些工作对红豆杉研究的重要意义,为红豆杉分子育种、功能基因挖掘及紫杉醇生物合成分子机理研究提供理论依据,并为今后的研究工作提供思路。Omics technology and multi-omics joint analysis have become common technical means in the research of animal and plant molecular biology. Omics technology based on transcriptomics, proteomics and metabonomics has been widely used in Taxus spp. various studies. This article reviews the applications of related genome, transcriptome, metabolome and proteome in recent years in the development of Taxus spp. molecular markers, taxol biosynthesis, functional genes, cold tolerance, and related metabolites related to taxol biosynthesis. The important significance of these works to the study of Taxus spp. is discussed, providing theoretical basis for Taxus spp.molecular breeding, functional gene mining and molecular mechanism of taxol biosynthesis, and providing ideas for future research work.

关 键 词：红豆杉(Taxus spp.) 转录组学 蛋白质组学 代谢组学 

分 类 号：S791.49[农业科学—林木遗传育种]