分散液相微萃取-高效液相色谱法测定婴幼儿血清中美罗培南的含量  

Determination of Meropenem in Infant Serum by High Performance Liquid Chromatography Coupled with Disperse Liquid-Liquid Microextraction

作　　者：郑凤家[1] 刘娜 王林 焦燕妮[1] 李蔚[1] 李凤华[1] 姜大峰[1] 王翠翠 ZHENG Fengjia;LIU Na;WANG Lin;JIAO Yanni;LI Wei;LI Fenghua;JIANG Dafeng;WANG Cuicui(Shandong Centre for Disease Control and Prevention,Jinan 250014,China;Shandong Institute for Food and Drug Control,Jinan 250101,China;Shandong Provincial Hospital,Jinan 250014,China)

机构地区：[1]山东省疾病预防控制中心,济南250014 [2]山东省食品药品检验研究院,济南250101 [3]山东省立医院,济南250014

出　　处：《理化检验（化学分册）》2022年第1期99-102,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：山东省健康长寿文化促进会新技术应用与健康促进专项科研基金(XJS2020-02)。

摘　　要：建立了分散液相微萃取-高效液相色谱法(HPLC)测定婴儿血清中美罗培南含量的方法。将经美罗培南药物治疗24h后的婴儿全血离心,移取婴儿血清样品100μL,加入50mg·L^(-1)甲硝唑(内标)溶液50μL和乙腈100μL,涡旋,离心,取全部上清液,加入二氯甲烷100μL,再次涡旋,离心,取上清液供HPLC分析。以Welch C_(18)色谱柱为固定相,以体积比为1:9的3-吗啡丙磺酸缓冲液(pH 7.0)-乙腈混合液为流动相进行等度洗脱。采用基质匹配混合标准溶液系列制作工作曲线,内标法定量。结果显示:美罗培南工作曲线的线性范围为0.25~100mg·L^(-1),检出限为0.06mg·L^(-1);对空白血清样品进行3个浓度水平的加标回收试验,回收率为98.6%~99.9%,测定值的相对标准偏差(RSD,n=6)为1.2%~2.0%。方法用于15名婴儿患者血清分析,美罗培南的检出量为19.2~98.9mg·L^(-1)。A method for determination of meropenem in infant serum by high performance liquid chromatography(HPLC)coupled with disperse liquid-liquid microextraction was established.50μL of 50mg·L^(-1)metronidazole(internal standard)solution and 100μL of acetonitrile were added into 100μL of infant serum sample from whole blood of the infant treated with moropenem for 24hafter centrifugation,and 10μL of dichloromethane was added into all the supernatant after vortex and centrifugation.Then supernatant was taken for HPLC analysis after vortex and centrifugation.Welch C_(18) column was used as the stationary phase,and a mixture of 3-morphine propyl acid buffer(pH 7.0)and acetonitrile at volume ratio of 1:9was used as the mobile phase for isocratic elution.Working curve was made by matrix matching mixed standard solution series,and internal standard method was used for quantitative analysis.As shown by the results,linear range of meropenem working curve was in the range of 0.25-100mg·L^(-1),with detection limit of 0.06mg·L^(-1).Recovery test was made on blank serum sample at 3concentration levels by standard addition method,giving results in the range of 98.6%-99.9%,and RSDs(n=6)of the determined values were ranged from 1.2%to 2.0%.This method has been applied for analysis of 15actual infant serum samples,with detection amounts of meropenem in the range of 19.2-98.9mg·L^(-1).

关 键 词：分散液相微萃取 高效液相色谱法 美罗培南 婴儿血清 

分 类 号：O657.7[理学—分析化学]