多尺度生命材料高压低温保存研究进展  

Research Progress of High-Pressure Cryopreservation for Multiscale Living Materials

作　　者：常铁 卫思源 安琪[1] 宋清雅 张兵[1] 赵刚[1] CHANG Tie;WEI Siyuan;AN Qi;SONG Qingya;ZHANG Bing;ZHAO Gang(Department of Electronic Engineering and Information Science,University of Science and Technology of China,Hefei 230052,China)

机构地区：[1]中国科学技术大学电子工程与信息科学系,合肥230052

出　　处：《真空科学与技术学报》2022年第2期83-93,共11页Chinese Journal of Vacuum Science and Technology

基　　金：国家自然科学基金项目(No.82172114);安徽省自然科学基金杰出青年科学基金项目(No.2108085J37)。

摘　　要：低温环境能够延缓生物体内新陈代谢进而抑制一系列生化反应的发生与进行,是诸多生物样本得以长期储存的重要手段。近年来,低温保存技术的成功应用,已直接、有效地推动了细胞、组织、器官、药物、食品等诸多领域的发展和进步,创造了显著的经济与社会效益,但实现高效健康的低温保存仍存在诸多挑战。非接触式的物理性压力增强技术是根据液态水的热力学和动力学性质改变其相转变行为,不会引入任何外源性物质,有望解决当前低温保存所存在的诸多难题。本文通过对高压冷冻以及定容冷冻两种低温保存方式进行综述,从其基本原理、研究进展、未来发展趋势进行总结概述,旨在促进高效、绿色、经济的生物低温保存技术。Cryopreservation technology can delay the metabolism of organisms and suppress the occurrence and progression of a series of biochemical reactions,which is a necessary means for long-term storage of many biological samples.In recent years,the successful application of cryopreservation technology has directly and effectively promoted the development and progress of cell,tissue,organ,medicine,food and other fields,creating remarkable economic and social benefits,but achieving efficient and healthy cryopreservation still remains many challenges.The non-contact physical pressure enhancement technology changes the phase transition behavior of water in term of the principle of thermodynamics and kinetics,it does not introduce any exogenous substances for biological samples,therefore it is expected to solve the current many critical problems existed in the field of cryopreservation.In this paper,two kinds of cryopreservation methods,high pressure freezing and constant volume freezing,were summarized from the aspect of basic principle,research progress,and future development trend,aiming to promote the development and progress of efficient,green,and economic cryopreservation technology.

关 键 词：低温保存 相变 高压冷冻 定容冷冻 

分 类 号：R318[医药卫生—生物医学工程]