森林植物多样性、树种重要值与土壤理化性质对球囊霉素相关土壤蛋白的影响  

Effects of plant species diversity,dominant species importance,and soil properties on glomalin-related soil protein

作　　者：陈胜仙 张喜亭 佘丹琦 张衷华 周志强 王慧梅 王文杰 Shengxian Chen;Xiting Zhang;Danqi She;Zhonghua Zhang;Zhiqiang Zhou;Huimei Wang;Wenjie Wang(Key Laboratory of Forest Plant Ecology(Ministry of Education),Key Laboratory of Forest Active Substance Ecological Utilization(Heilongjiang Province),Northeast Forestry University,Harbin 150040;Key Laboratory of Wetland Ecology and Environment,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Changchun 130102)

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,黑龙江省林源活性物质生态利用重点实验室,哈尔滨150040 [2]中国科学院东北地理与农业生态研究所,长春130102

出　　处：《生物多样性》2022年第2期47-59,共13页Biodiversity Science

基　　金：国家自然科学基金(41730641,41877324);中央高校基本科研业务费专项资金(2572021DT03,2572018AA19);黑龙江省头雁计划项目的支持。

摘　　要：球囊霉素相关土壤蛋白(glomalin-related soil protein,GRSP)在土壤物理结构调节和土壤碳库稳定性中发挥着重要作用,但植物多样性和优势种如何影响GRSP还缺乏系统性研究。本研究依托东北林业大学哈尔滨实验林场的72块样地,对1 m深土壤剖面分5层采样,测定土壤易提取球囊霉素(easily extractable GRSP,EEG)、总提取球囊霉素(total GRSP,TG)及土壤理化性质,并同时计算植物多样性指数及优势种重要值(importance value,IV),进一步通过相关分析和冗余排序分析判断影响GRSP的主要因素与贡献。结果表明:(1)在整个土壤剖面上均表现为TG和EEG与土壤有机碳(SOC)正相关,在部分土层深度与全氮(total nitrogen,TN)和含水量(moisture content,MC)正相关,而与电导率(electrical conductivity,EC)和pH值负相关。(2)部分土层TG和EEG与黑皮油松(Pinus tabuliformis var.mukdensis)、樟子松(P.sylvestris var.mongolica)、胡桃楸(Juglans mandshurica)、黄檗(Phellodendron amurense)、榆树(Ulmus pumila)优势种重要值显著相关,表现为黑皮油松重要值越高,而黄檗、榆树重要值越小,越有利于EEG的积累,并且伴随EEG-C/SOC(EEG中C占SOC比例)增加、EEG/TG增大;群落中胡桃楸、黄檗、榆树更有利于TG积累,黑皮油松、落叶松(Larix gmelinii)、樟子松不利于TG的积累。(3)植物Simpson指数、Shannon-Wiener指数、物种丰富度与EEG、TG、EEG/TG无显著相关性,而与EEG-C/SOC、EEG-N/TN(EEG中N占TN的比例)、TG-C/SOC(TG中C占SOC比例)、TG-N/TN(TG中N占TN的比例)显著负相关;土壤EEG/TG和EEG-N/TN与植物均匀度指数显著正相关,在1 m土壤不同土层趋势类似。(4)方差分解分析表明:生物因子对GRSP变化的解释率是20.2%,土壤理化因子解释率为7.8%,而生物因子中植物优势种重要值的解释率最大(16.4%),而植物物种多样性指数解释率仅为0.4%。冗余排序发现常绿针叶树种(黑皮油松和樟子松)越多且阔叶树种越少时,GRSP含量Aims Glomalin related soil protein(GRSP)is a type of glycoprotein produced by arbuscular mycorrhizal fungi,playing a vital role in the regulation of soil physical structure and soil carbon stability.Previous studies have demonstrated the effects of soil physico-chemical properties on GRSP accumulation.To date,there is a lack of systematic research on how plant species composition of diversity and dominance affecting GRSP accumulation.Methods A total of 72 plots in Harbin Experimental Forest Farm of Northeast Forestry University were surveyed in this study.The soil profile at 1 m depth was divided into five layers for soil sampling.Tree species diversity indices(Richness,Shannon-Wiener index,Simpson index,and evenness index)were calculated.The importance values(IVs)were calculated for dominant tree species according to the relative abundance,species frequency,and cross-area ratio to total stem cross-area.Soil physico-chemical properties were measured,including soil organic carbon(SOC),total nitrogen(TN),soil moisture content(MC),electrical conductivity(EC),and pH.All soil samples were assayed for easily extractable GRSP(EEG),total GRSP(TG),EEG/TG,EEG-C/SOC,and TG-C/SOC.The association between GRSP traits and soil physicochemical properties,IVs of dominant trees,and tree species diversity were ordinated using redundancy ordination(RDA)and variation partitioning analysis.Results(1)TG and EEG correlated positively with SOC throughout the entire soil profile.The same was true with TN and MC at some soil depths,but the correlation was negative with EC and pH values.(2)In most soil layers,the greater EEG accumulation with higher EEG-C/SOC(proportion of C in EEG to SOC)and EEG/TG accompanied with the higher IV for Pinus tabulaeformisvar.mukdensis,but smaller IVs for Phellodendron amurense and Ulmus pumila.In contrast,the greater TG accumulation aligned with the higher IVs in Juglans mandshurica,P.amurense,and U.pumila but lower IVs in P.tabulaeformis var.mukdensis,Larix gmelinii and Pinus sylvestris var.mongolica.(3)The

关 键 词：球囊霉素相关土壤蛋白 物种多样性 优势种重要值 土壤理化性质 冗余排序和方差分解分析 

分 类 号：S718.5[农业科学—林学]