一株兔出血症病毒变异毒株的分离与鉴定  

Isolation and identification of a mutant strain of RHDV

作　　者：吴忆春[1] WU Yichun(Biological Engineering College,Binzhou Polytechnic,Binzhou 256603,China)

机构地区：[1]滨州职业学院生物工程学院,山东滨州256603

出　　处：《黑龙江畜牧兽医》2022年第4期78-81,137,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：山东省高等学校科技计划项目(J18KA169);滨州职业学院科研项目(2020yjkt12)。

摘　　要：2021年3月份河南省某养兔场肉兔疑似感染兔病毒性出血症病毒(Rabbit hemorrhagic disease virus, RHDV),为了分离鉴定肉兔感染毒株,并对所分离毒株特性进行研究,试验对兔场送检的病死兔进行解剖、细菌与病毒分离、RT-PCR检测、纯净性检测、血凝性测定,并进一步进行半数致死量(LD;)、传统疫苗免疫兔攻毒保护试验和免疫原性测定。结果表明:病死兔剖检可见肝脏、心脏、肺脏等实质内脏器官广泛出血。病料未分离到细菌,RT-PCR检测鉴定感染病毒为RHDV;用分离株注射非免疫健康家兔后,兔48 h内死亡,症状表现和剖检病变与兔出血症相似;将所分离RHDV命名为HN株,其对人"O"型红细胞无血凝性,对兔的LD_(50)为1×10^(-6.77)/mL;灭活后免疫兔,能抵抗RHDV HN株攻击。说明养兔场肉兔感染了RHDV,且成功分离鉴定得到一株变异型RHDV,其具有良好的免疫原性。In March 2021, Rabbit hemorrhagic disease virus(RHDV) infection was suspected in meat rabbits in a rabbit farm in Henan Province.In order to isolate and identify the infected strain from meat rabbits, and to study the characteristics of the isolated strain, the test dissected sick and dead rabbits being sent from the rabbit farm, and carried out bacteria and virus separation, RT-PCR detection, purity detection, hemagglutination test determination;and LD;, traditional vaccine immunization rabbit challenge protection test and immunogenicity determination were further conducted. The results showed that the main manifestations of dead rabbits were extensive bleeding in liver, heart, lung and other parenchymal organs;no bacteria was isolated in diseased samples;RT-PCR detection identified the infected virus as RHDV. After injection of non-immunization healthy rabbits, the rabbits died within 48 hours, and the symptoms and necropsy lesions were similar to rabbit hemorrhagic disease. The isolated RHDV was named HN strain, which had no hemagglutination to human “O” red blood cells, and its LD_(50) to rabbits was 1×10^(-6.77)/mL. Immunized rabbits after inactivation could resist the attack of RHDV HN strain. The results suggested that the meat rabbits in the rabbit farm were infected with RHDV, and a variant RHDV was successfully isolated and identified, which had good immunogenicity.

关 键 词：兔出血症病毒 变异毒株 分离 鉴定 攻毒保护性试验 免疫原性测定 

分 类 号：S852.65[农业科学—基础兽医学]