构树叶粉抗鸡柔嫩艾美耳球虫感染的试验研究  

Experimental study on the resistance of Broussonetia papyrifera leaf powder against Eimeria tenella infection in chicken

作　　者：徐百昌 郝凯源 吕菲菲 方紫依 沈水宝 司红彬[1] XU Baichang;HAO Kaiyuan;LYU Feifei;FANG Ziyi;SHEN Shuibao;SI Hongbin(College of Animal Science and Technology,Guangxi University,Nanning 530004,China)

机构地区：[1]广西大学动物科学技术学院,南宁530004

出　　处：《黑龙江畜牧兽医》2022年第2期107-112,140,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：广西重点研发计划项目(桂科AB19245037);南宁市重点研发计划项目(20202088);北海市科学研究与技术开发项目(北科合202082037);广西大学“大学生创新创业训练计划”项目(202010593312)。

摘　　要：为了评价构树叶对鸡球虫病的防治效果,试验采用人工感染球虫的方法,以1日龄三黄鸡为研究对象,共分为4组,分别为正常组(正常饲喂)、感染组(感染球虫不治疗)、构树叶粉组(感染球虫+添加6%构树叶粉)、地克珠利组(感染球虫+0.05%地克珠利),每组3个重复,每个重复5只鸡。除正常组外,其他各组于14日龄经口人工感染7.5×10^(4)个球虫孢子化卵囊,观察雏鸡的临床症状;在21日龄时处死雏鸡并进行盲肠病变、血清抗氧化能力、肠道紧密连接蛋白相关基因mRNA相对表达量等相关指标的检测。结果表明:构树叶粉组的抗球虫指数为156.69,可达到中等抗球虫效果;与感染组相比,构树叶粉组可以提高雏鸡存活率,缓解感染雏鸡体重下降,显著降低盲肠OPG(P<0.05);改善柔嫩艾美耳球虫感染造成的盲肠组织损伤,提高雏鸡的抗氧化能力,促进肠道紧密连接相关蛋白基因(ZO-1、Occludin)的表达。说明在日粮中添加6%构树叶粉可以在一定程度上防治雏鸡柔嫩艾美耳球虫病。In order to evaluate the control effect of Broussonetia papyrifera leaves on chicken coccidiosis, the experiment adopted the method of artificial infection with coccidia. The one-day-old three-yellow chickens were divided into four groups, namely the normal group(normal feeding), the infected group(infected with coccidia without treatment), and the leaf powder group(infected with coccidia + 6% Broussonetia papyrifera leaf powder added), diclazuril group(infected with coccidia + 0.05% diclazuril);three repetitions per group, and five chickens per repetition. Except for the normal group, the other groups were orally infected with 7.5×10^(4)coccidia sporulated oocysts at the age of 14 days, and the clinical symptoms of the chicks were observed. The chicks were killed at 21 days of age and tested for cecal lesions, serum antioxidant capacity, intestinal tight junction proteins related genes mRNA relative expression and other related indicators. The results showed that the anti-coccidial index of the Broussonetia papyrifera leaf powder group was 156.69, which could achieve a moderate anti-coccidial effect. Compared with the infected group, the Broussonetia papyrifera leaf powder group could improve the survival rate of the chicks, slow down the weight loss of the infected chicks, and significantly reduce the OPG of the cecum(P<0.05);it could improve the cecal tissue damage caused by Eimeria tenella infection, improve the antioxidant capacity of chicks, and promote the expression of intestinal tight junction related proteins genes(ZO-1, Occludin). The results suggested that adding 6% Broussonetia papyrifera leaf powder to the diet could prevent and control coccidiosis in chicks to a certain extent.

关 键 词：杂交构树叶 柔嫩艾美耳球虫 防治效果 抗氧化 肠道紧密连接 

分 类 号：S853.32[农业科学—临床兽医学]