2017-2019年河南省新乡市疑似HIV感染样本确证试验结果分析  

Analysis of confirmatory test results of suspected HIV infected samples in Xinxiang city,Henan province,2017-2019

作　　者：程栋[1] 徐璐 薛秀娟[2] 刘春华[2] 于贺军[1] 付惠成[1] CHENG Dong;XU Lu;XUE Xiujuan;LIU Chunhua;YU Hejun;FU Huicheng(Xinxiang Center for Disease Control and Prevention,Xinxiang,Henan 453000,China;不详)

机构地区：[1]新乡市疾病预防控制中心,河南新乡453000 [2]河南省疾病预防控制中心

出　　处：《河南预防医学杂志》2022年第1期84-86,共3页Henan Journal of Preventive Medicine

基　　金：HIV抗原抗体联合快速检测试剂在基层检测点的推广应用项目(201602311)。

摘　　要：目的分析2017-2019年新乡市疑似艾滋病病毒(HIV)感染样本的确证试验结果,为艾滋病检测工作提供依据。方法参照《全国艾滋病检测技术规范(2015年修订版)》,采用蛋白免疫印迹法进行确证试验,按照试剂说明书进行条带分析和结果判定。结果2017-2019年新乡市各筛查实验室送检疑似HIV感染样本957例,经过确证试验,被判定为HIV抗体阳性546例(57.05%),HIV抗体阴性182例(19.02%),HIV抗体不确定229例(23.93%)。不同年份间HIV抗体阳性、阴性、不确定的检出率差异具有统计学意义(P<0.05);男性HIV抗体阳性率为65.54%,高于女性的39.09%(P<0.05);不同来源的样本HIV抗体阳性率差异具有统计学意义(P<0.05),其中最高的来源于自愿咨询(VCT)为79.69%,最低的来源于采供血机构为3.54%。HIV抗体阳性样本中gp160出现频率最高(100.00%),其次为gp120(99.82%)。结论应扩大检测覆盖面,提升VCT等来源的高危人群的检测率,为早发现早治疗提供实验室依据;针对HIV抗体不确定样本,尽可能加做CD4^(+)T淋巴细胞检测和病毒载量进行辅助诊断,缩短HIV疑似感染者的诊断时间。Objective To analyze the results of confirmatory test of suspected HIV infection(HIV)in Xinxiang from 2017 to2019,and to provide evidence for HIV testing.Methods According to the national AIDS testing technology standard suspected HIV infection(Revised Edition 2015),the Western blot method was used to confirm the test,and the strip analysis and result determination were carried out according to the reagent instructions.Results From 2017 to 2019,957 suspected HIV infection samples were sent by screening laboratories in Xinxiang City.After confirmatory test,546 cases(57.05%)were determined to be HIV antibody positive,182 cases(19.02%)were HIV antibody negative,and 229 cases(23.93%)were HIV antibody uncertain.The positive,negative and uncertain detection rates of HIV antibody in different years were statistically significant(P<0.05);In terms of gender,the positive rate of HIV antibody in men was 65.54%,higher than in women(39.09%),and the difference between men and women was statistically significant(P<0.05);in terms of sample sources,the highest HIV antibody positive rate came from voluntary counseling(VCT),which was 79.69%,and the lowest from blood collection and supply institutions,which was 3.54%,and the difference was statistically significant(P<0.05).The frequency of gp160 in HIV antibody positive samples was the highest,followed by gp120.Conclusion For VCT and other key groups,we should further strengthen the publicity of AIDS prevention knowledge,expand the coverage of detection,and improve the detection rate in high-risk groups,and provide laboratory evidence for early detection and early treatment.On the other hand,for uncertain samples,CD4^(+)T lymphocyte detection and viral load should be added as much as possible for auxiliary diagnosis,and to shorten the diagnosis time of people suspected of HIV infection.

关 键 词：艾滋病病毒 确证试验 条带 抗体 

分 类 号：R512.91[医药卫生—内科学]