间接竞争酶联免疫分析方法检测青霉素G  

The indirect competitive enzyme-linked immunosorbent assay for penicillin G

作　　者：王玉珍[1] 田茂忠[1] 张小玲 温雅琼[1] 王珠倩 WANG Yu-zhen;TIAN Mao-zhong;ZHANG Xiao-ling;WEN Ya-qiong;WANG Zhu-qian(College of Chemistry and Chemical Engineering,Shanxi Datong University,Datong 037009,China;Chongqing Kaizhou District Maternal and Child Health Hospital,Chongqing 405400,China)

机构地区：[1]山西大同大学化学与化工学院,山西大同037009 [2]重庆市开州区妇幼保健院,重庆405400

出　　处：《化学研究与应用》2022年第3期657-661,共5页Chemical Research and Application

基　　金：山西省高等学校科技创新项目(2019L0758)资助;山西省应用基础研究计划项目(201901D211436)资助;山西大同大学大学生创新创业训练计划项目(XDC2020218)资助。

摘　　要：本研究介绍了青霉素G残留的危害,并建立了间接竞争酶联免疫分析方法(Enzyme-linked Immunosorbent Assay,ELISA)检测牛奶类样品中青霉素G的残留量。首先制备出青霉素G的单克隆抗体,在最优实验条件下,青霉素G浓度范围在1~1000 ng·mL^(-1)内,所建立方法的灵敏度(IC_(50))为17.33 ng·mL^(-1),检出限为0.75 ng·mL^(-1)。该抗体与苄星青霉素交叉反应率为30.09%,而与氨苄青霉素、羧苄青霉素、阿莫西林、先锋霉素、头孢噻肟钠均无交叉反应。牛奶类样品中青霉素G的加标回收率为81.70%~99.08%,相对标准偏差为1.82%~11.41%。以上结果表明,本研究建立的间接竞争酶联免疫吸附分析方法有较高的灵敏度和较强的特异性,可以用于青霉素G的检测。The harms of penicillin G residues are here introduced and determined with an indirect competitive enzyme-linked immunosorbent assay(ELISA).At optimal experimental conditions,the standard curve was constructed in concentrations of 1~1000 ng·mL^(-1).The 50%inhibitory concentration(IC_(50))was 17.33 ng·mL^(-1)and the detection limit was 0.75 ng·mL^(-1).The antibody has a relatively high cross-reactivity rate(30.09%)with benzathine penicillin,whereas no cross-reactivity rate was found with ampicillin,carboxy benzylpenicillin,amoxicillin,cephalothin and cefotaxime.Acceptable recovery rates of 81.70%~99.08%and relative standard deviation of 1.82%~11.41%were obtained in milk samples.The mAb-based ELISA proven to be a feasible quantitative/screening method for penicillin G in milk samples with the properties of high sensitivity,specificity,simplicity of sample pretreatment,high sample throughput and low expense.

关 键 词：青霉素G 单克隆抗体 酶联免疫吸附分析方法(ELISA) 样品 

分 类 号：O652.1[理学—分析化学]