苗药痛风汤方剂对痛风性关节炎大鼠炎症反应及NALP3炎性体信号的影响  

Effect of Miao medicine gout decoction on inflammatory reaction and NALP3 in flammasome pathway of rats with gouty arthritis

作　　者：樊丰夷[1] 蓝天座[1] 于黔[1] 吴欣[1] 杨旸[1] 杨静[1] FAN Fengyi;LAN Tianzuo;YU Qian;WU Xing;YANG Yang;YANG Jing(Department of Nephrology, Guiyang First People's Hospital, Guiyang 550002, China)

机构地区：[1]贵阳市第一人民医院肾内科,贵州贵阳550000

出　　处：《西部医学》2022年第3期375-380,385,共7页Medical Journal of West China

基　　金：贵阳市科技计划项目(2019-9-8)。

摘　　要：目的探讨苗药痛风汤方剂对痛风性关节炎(GA)大鼠炎症反应及NALP3炎性体信号的影响。方法40只健康雄性SD大鼠随机分为5组,即空白组、模型组、双氯芬酸钠肠溶片组、苗药痛风汤方剂低、高剂量组,每组8只。采用踝关节腔内一次性注射尿酸钠构建急性GA大鼠模型,造模成功后,空白组、模型组给予生理盐水,双氯芬酸钠肠溶片组及苗药痛风汤方剂低、高剂量组分别按相应剂量给予双氧芬酸钠肠溶片及苗药痛风汤方剂连续灌胃5 d,处死大鼠,取膝关节滑膜组织。采用HE染色观察滑膜组织病理变化;ELISA检测血清TNF-α、IL-1β、IL-8、β-gal、NAG、SOD、MDA水平;qRT-PCR检测滑膜组织NALP3、Caspase-1、ASC mRNA表达;Western Blot检测滑膜组织NALP3、Caspase-1、pro-IL-1β蛋白表达。结果与空白组相比,模型组滑膜组织衬里下层大量炎性细胞浸润,关节肿胀指数明显增加,TNF-α、IL-1β、IL-8、β-gal、NAG、MDA、NALP3、Caspase-1、ASC、pro-IL-1β水平明显升高,SOD水平明显降低(均P<0.05);与模型组比较,苗药痛风汤方剂高剂量组TNF-α、IL-1β、IL-8、β-gal、NAG、MDA、NALP3、Caspase-1、ASC、pro-IL-1β水平明显降低,SOD水平明显升高(均P<0.05)。结论苗药痛风汤方剂可减轻GA大鼠组织炎症程度,下调NALP3及炎性细胞因子的表达,可能有抑制急性痛风性关节炎发作的重要作用。Objective To explore the effect of Miao medicine gout decoction on inflammatory reaction and NALP3 inflammasome pathway of gouty arthritis.Methods A total of 40 healthy SD rats were randomly divided into control group,model group,diclofenac sodium enteric-coated tablet group,low-dose and high-dose Miao Yao gout decoction group.The rat model of acute gouty arthritis was successfully established via a one-time injection of sodium urate into ankle joint cavity.After successful modeling,the rats in the control group and model group were given physiological salt water,diclofenac sodium enteric-coated tablets group and low-dose and high-dose Miao medicine gout decoction groups respectively according to the corresponding dose for 5 days.The rats were killed,and the synovium of knee joint was taken.The pathological changes of synovial tissue were observed by HE staining,the serum levels of TNF-α,IL-1β,IL-8,β-gal,NAG,SOD and MDA were detected by ELISA,and the expression of NALP3,Caspase-1 and ASC mRNA in synovial tissue was detected by qRT-PCR.Western Blotting was used to detect the expression of NALP3,Caspase-1 and pro-IL-1βprotein in synovial tissue.Results Compared with the control group,a large number of inflammatory cells infiltrated the lower layer of synovial tissue,and the joint swelling index increased significantly in the model group,and the levels of TNF-α,IL-1β,IL-8,β-gal,NAG,MDA,NALP3,Caspase-1,ASC and pro-IL-1βincreased significantly,while the level of SOD decreased significantly in the model group(P<0.05).Compared with the model group,the levels of TNF-α,IL-1β,IL-8,β-gal,NAG,MDA,NALP3,Caspase-1,ASC and pro-IL-1βin the high dose group of Miao medicine gout decoction decreased significantly,while the level of SOD increased significantly(P<0.05).Conclusion Miao medicine gout decoction can reduce the degree of inflammation with gout rat model,and down-regulate the expression of NALP3 and inflammatory factors,which may play an important role in inhibiting the attack of acute gouty arthritis.

关 键 词：痛风性关节炎 苗药痛风汤方剂 双氯芬酸钠肠溶片 NALP3 

分 类 号：R285.5[医药卫生—中药学] R589.7[医药卫生—中医学]