北江荛花茎段离体快速繁殖与叶片再生体系构建  

In vitro Propagation of Wikstroemia monnula from Stem and Leaf

作　　者：雷佩雯 干诗颖 孙家怡 彭思娴 栾牧 高培军[1,2] LEI Pei-wen;GAN Shi-yin;SUN Jia-yi;PENG Si-xian;LUAN Mu;GAO Pei-jun(State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,China;College of Environment and Biotechnological,Zhejiang A&F University,Hangzhou 311300,China)

机构地区：[1]浙江农林大学省部共建亚热带森林培育国家重点实验室,浙江杭州311300 [2]浙江农林大学林业与生物技术学院,浙江杭州311300

出　　处：《浙江林业科技》2022年第2期8-14,共7页Journal of Zhejiang Forestry Science and Technology

摘　　要：开化纸产自浙江省的开化县,纸张细腻、柔软、不易折毁、可久藏,是现代古籍修复重要用纸。开化纸核心造纸原料是荛花属Wikstroemia植物,目前国内外尚无人工林种植,主要依靠采挖有限的野生资源和从菲律宾进口干料,这已经严重影响开化纸的产业化发展。为了实现荛花种苗快速繁育,本研究以北江荛花Wikstroemia monnula当年生幼嫩茎段和叶片为外植体进行组织培养,探索北江荛花组织培养最佳外植体消毒方式,外植体诱导和分化、增殖、壮苗培养的最佳培养基配方。结果表明,用0.25%NaClO溶液浸泡消毒10 min为最佳消毒方式;腋芽诱导培养基WPM+0.01 mg·L^(-1)6-BA可直接诱导茎段获得不定芽,腋芽诱导率达45.3%;愈伤组织诱导培养基MS+4 mg·L^(-1) TDZ+2 mg·L^(-1)2,4-D的诱导效果最好,叶片愈伤组织诱导率可达65.1%,且在分化培养基MS+2.5 mg·L^(-1) TDZ+1.0 mg·L^(-1) NAA中分化出不定芽,诱导率为64.9%;增殖培养基WPM+0.1 mg·L^(-1)6-BA+0.1 mg·L^(-1) NAA培养的组培苗增殖较好,增殖系数可达4.84;壮苗培养基WPM+0.1 mg·L^(-1)6-BA+0.1 mg·L^(-1) NAA+1000 mg·L^(-1) PVP可提高增殖系数,达5.42。上述研究结果为荛花种苗快速繁育提供了重要的技术支持。Tissue culture of current year leaf and stem of Wikstroemia monnula was conducted in Hangzhou,Zhejiang province.Experiments were carried out on different explant disinfections,medium for induction,differentiation,multiplication and cultivation.The results showed that the best disinfection method was to soak the explants in 0.25%sodium hypochlorite solution for 10 minutes.Induction medium of WPM+0.01 mg/L of 6-BA could introduce bud from 45.3%of the stem.MS+4 mg/L of TDZ+2 mg/L of 2,4-D had the highest callus induction ratio of 65.1%,and then MS+2.5 mg/L of TDZ+1.0 mg/L of NAA had bud induction of 64.9%.WPM+0.1 mg/L of 6-BA+0.1 mg/L of NAA had multiplication coefficient of 4.84,while WPM+0.1 mg/L of 6-BA+0.mg/L of NAA+1000 mg/L of PVP could increase multiplication coefficient to 5.42.

关 键 词：北江荛花 愈伤组织 组织培养 快速繁殖 开化纸 茎段 叶片 

分 类 号：Q943.1[生物学—植物学]