The chromosome-level genome assembly of Astragalus sinicus and comparative genomic analyses provide new resources and insights for understanding legume-rhizobial interactions  

作　　者：Danna Chang Songjuan Gao Guopeng Zhou Shuhan Deng Jizeng Jia Ertao Wang Weidong Cao 

机构地区：[1]Key Laboratory of Plant Nutrition and Fertilizer,Ministry of Agriculture and Rural Affairs,Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China [2]Graduate School,Chinese Academy of Agricultural Sciences,Beijing 100081,China [3]College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095,China [4]Glbizzia Biological Science and Technology,Co,Ltd,Beijing,China [5]Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China [6]National Key Laboratory of Plant Molecular Genetics,Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences,Institute of Plant Physiology and Ecology,Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China

出　　处：《Plant Communications》2022年第2期62-75,共14页植物通讯（英文）

基　　金：This research was financially supported by China Agricultural Research System of MOF and MARA(CARS-22);Chinese Outstanding Talents Pro-gram in Agricultural Science,Agricultural Science and Technology Innova-tion Program of CAAS;China National Crop Germplasm Resources Platform for Green Manure(NICGR-2021-19).

摘　　要：The legume species Astragalus sinicus(Chinese milk vetch[CMV])has been widely cultivated for centuries in southern China as one of the most important green manures/cover crops for improving rice productivity and preventing soil degeneration.In this study,we generated the first chromosome-scale reference genome of CMV by combining PacBio and Illumina sequencing with high-throughput chromatin conformation capture(Hi-C)technology.The CMV genome was 595.52 Mb in length,with a contig N50 size of 1.50 Mb.Long terminal repeats(LTRs)had been amplified and contributed to genome size expansion in CMV.CMV has undergone two whole-genome duplication(WGD)events,and the genes retained after the WGD shared by Papilionoideae species shaped the rhizobial symbiosis and the hormonal regulation of nodulation.The chalcone synthase(CHS)gene family was expanded and was expressed primarily in the roots of CMV.Intriguingly,we found that resistance genes were more highly expressed in roots than in nodules of legume species,suggesting that their expression may be increased to bolster plant immunity in roots to cope with pathogen infection in legumes.Our work sheds light on the genetic basis of nodulation and symbiosis in CMV and provides a benchmark for accelerating genetic research and molecular breeding in the future.

关 键 词：Astragalus sinicus GENOME chalcone synthase(CHS)gene R genes 

分 类 号：Q943.2[生物学—植物学]